human embryonic kidney, 64 day (ATCC® 87258)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: DM Kurnit

Permits and Restrictions
Permits and Restrictions

View Permits

Designations human embryonic kidney, 64 day
Species Homo sapiens, human
Depositors DM Kurnit
Origin
embryonic kidney, 64 day
Applications
This host is strongly recommended for screening and amplification of the library.
The host DA1061 (ATCC 47088) suppresses the formation of large non-lambda phage plaques.
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaZAPII
Intact vector size: 40.820
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: SacI NotI XbaI SpeI EcoRI XhoI
Polylinker sites: SacI NotI XbaI SpeI EcoRI XhoI
Construction: pBluescript SK-
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1, lambda
Cross references:
Vector
Tissue: embryonic kidney, 64 day
DNA: cDNA
Digest: Complete
Insert size range: 0.3 - 0.5 kb
Coverage: Clones for "complete" coverage: 1000000.0
Biosafety Level 1
Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
Comments
The host DA1061 (ATCC 47088) suppresses the formation of large non-lambda phage plaques. This host is strongly recommended for screening and amplification of the library.
References

Buraczynska MJ, et al. Construction of human embryonic cDNA libraries: HD, PKD1 and BRCA1 are transcribed widely during embryogenesis. Cytogenet. Cell Genet. 71: 197-202, 1995. PubMed: 7656596

David M Kurnit, personal communication

Product Sheet
Product Sheet
Product Sheet