Human embryonic kidney, 57 day (ATCC® 87257)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: DM Kurnit, Howard Hughes Medical Institute

Permits and Restrictions

View Permits

Designations Human embryonic kidney, 57 day
Species Homo sapiens, human
Depositors DM Kurnit, Howard Hughes Medical Institute
Origin
embryonic kidney, 57 day
Applications
This host is strongly recommended for screening and amplification of the library.
The host DA1061 (ATCC 47088) suppresses the formation of large non-lambda phage plaques.
Vector Vector name: lambdaZAPII
Intact vector size: 40.820
Type of vector: Phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: SacI NotI XbaI SpeI EcoRI XhoI
Features:
Insert detection: lacZ’
Marker: ampR
Promoters: lac, T3, T7
Replicons: pMB1, f1, lambda
Insert Genome: Human
Tissue: Embryonic kidney, 57 day
DNA: cDNA
Insert size: 0.3 - 0.5 kb
Insert end modification: EcoRI adaptor
Insert end modification: EcoRI adaptor
Number of independent recombinants: 1 x 106
Nonrecombinants (%): 21.70
Titer: 3.8 x 107 pfu/mL
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Frozen bacteria-free lysate
Comments
The host DA1061 (ATCC 47088) suppresses the formation of large non-lambda phage plaques. This host is strongly recommended for screening and amplification of the library.
References

Buraczynska MJ, et al. Construction of human embryonic cDNA libraries: HD, PKD1 and BRCA1 are transcribed widely during embryogenesis. Cytogenet. Cell Genet. 71: 197-202, 1995. PubMed: 7656596

David M Kurnit, personal communication