pOSEX4 (ATCC® 87213)

Applications: expression vector  /  Depositors: E Bremer

Permits and Restrictions

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Designations pOSEX4
Depositors E Bremer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.34
Vector: pOSEX4 (plasmid)
Promoters: Promoter for expression proU
Construction: pOSEX2
Construct size (kb): 3.34
Features: marker(s): ampR
promoter for expression: proU
replicon: pMB1
MCS: BamHI...HindIII
transcription terminator: rrnB T1
transcription terminator: rrnB T2
coding sequence: proV 5' sequence
expression vector
Restriction digests of the clone give the following sizes (kb): BamHI--3.3; BglI--1.9, 1.4; HindIII--3.3.
Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride.
Vector does not provide a ribosome binding site or initiation codon.
Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter.
This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor.
Vector constructed from pOSEX2 (ATCC 87211) by deletion of a portion of the pBR322 backbone, including the copy control region.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Herbst B, et al. pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli. Gene 151: 137-142, 1994. PubMed: 7828862

Erhard Bremer, personal communication

Shipped freeze-dried
Shipping Information

 Freeze dried plasmid in E. coli