pLDR20 [WM2193] (ATCC® 87205)

Applications: contains easily purifiable cassette(s) for construction cI857 - PR cassette  /  Depositors: W Messer

Permits and Restrictions

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Designations pLDR20 [WM2193]
Depositors W Messer
Biosafety Level 1
Vector Information
Size (kb): 3.2170000076293950
Vector: pLDR20 (plasmid)
Construction: pOU71, pT7-7
Marker(s):ampR
Construct size (kb): 3.217000007629395
Features: E. coli promoter cassette: cI857 - PR cassette
marker(s): ampR
replicon: pMB1
repressor gene: cI857
MCS: ClaI...XhoI
Applications
contains easily purifiable cassette(s) for construction cI857 - PR cassette
Comments
Restriction digests of the clone give the following sizes (kb): BglI--3.35; HindIII--3.4; XhoI--2.1, 1.15.
Helper plasmid with an XhoI promoter cassette containing the gene for cI857 and the lambda PR promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped frozen
Shipping Information

 Frozen glycerol stock of  E. coli containing the plasmid

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87205