2P2 (ATCC® 87189)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: F Kao

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Designations 2P2
Species Homo sapiens, human
Depositors F Kao
Vector
Chromosome: 2p23-p21
DNA: genomic
Digest: Complete
Insert size range: 50 - 600 bp
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Approximately 45% of the clones contain repetitive sequences. Of 94 single copy clones tested, 60 (64%) hybridized to chromosome 2.
Constructed by microdissecting fragments from 20 chromosomes, treating with proteinase K, extracting with phenol, ligating to an MboI linker-adaptor, amplifying by PCR, digesting with MboI, and ligating to pUC19.
References

Kao FT, et al. A region-specific microdissection library for human chromosome 2p23-->p21 and the analysis of an interstitial deletion of 2p21. Cytogenet. Cell Genet. 68: 17-18, 1995. PubMed: 7956351

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