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pBSL141

87146

Plasmid useful for vector construction and contains a gentamicin acetyltransferase (aacC1, gmR) cassette flanked by multiple cloning sites duplicated in tandem orientation.This was constructed by cloning a 0.8 kb mLuI fragment containing the complete coding sequence for gentamycin acetyltransferase (aacC1) from Tn1696 into the mLuI site of the sBSL98 phagemid. The sequence for the aacC1 gene has Genbank accession number X15852.
Organism
Tn1696
Clone type
Vector
Applications
Molecular biology
Product format
Frozen
Shipping information
Escherichia coli containing the phagemid in glycerol stock
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
contains easily purifiable cassette(s) for construction gentamicin acetyltransferase

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): MluI--3.1, 0.8; BamHI--2.9, 1.0; NotI--2.9, 1.0.
Problems have been reported when using DH5alpha as a host for multicopy plasmids bearing Gm and Tc, but not when TG1 is used.
Plasmid useful for vector construction. Contains a gentamicin acetyltransferase (aacC1, gtmR) cassette flanked by multiple cloning sites duplicated in tandem orientation.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
3.885999917984009
Vector name
pBSL141 (phagemid)
Construction
pBluescript
Coding sequence
aacC1 (gtmR)
Markers
gtmR; ampR
MCS
KpnI...SacI MluI; MluI KpnI...SacI
Promoters
In vitro transcription: T7 (phi10)
Replicon
f1; pBM1

Insert information

Insert size (kb)
0.80000000000000004
Type of DNA
genomic
Target gene
gentamicin acetyltransferase
Gene product
gentamicin acetyltransferase [aacC1]

Handling information

Medium
Temperature
37°C
Handling notes
Restriction digests of the clone gave the following sizes   (in kb):  mLuI – 3.1, 0.8 ; BamHI – 2.1, 1.0 ; NotI – 2.9, 1.0.                                                     –ATCC Staff    

History

Depositors
MF Alexeyev
Special collection
NCRR Contract
Cross references
GenBank X15852

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Mikhail F Alexeyev, personal communication

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