pBSL14 (ATCC® 87127)

Applications: contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance  /  Depositors: MF Alexeyev

Designations pBSL14
Depositors MF Alexeyev
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 3.9000000953674320
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: cosmid
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752
Vector: pBSL14 (plasmid)
Construction: pUC18, pK18
Marker(s):ampR,kanR
Construct size (kb): 3.900000095367432
Features: marker(s): ampR
marker(s): kanR
replicon: pMB1
MCS: HindIII...EcoRI
MCS: HindIII...NheI
Applications
contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 1.1; HindIII--2.8, 1.1; BamHI--2.7, 1.2.
The nptII gene was modified by site-directed mutagenesis to remove PstI and SphI sites.
Plasmid with an easily purified kanR cassette (flanked by direct repeats of the pUC18 multiple cloning site) for use in construction.
Media ATCC® Medium 1948: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 20 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF. Three kanamycin resistance gene cassettes with different polylinkers. BioTechniques 18: 52-55, 1995. PubMed: 7702853

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map