pJC40 (ATCC® 87114)

Applications: encodes removable tag for protein isolationexpression vectorvector for generating nested deletions of cloned inserts  /  Depositors: J Clos

Permits and Restrictions

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Designations pJC40
Depositors J Clos
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.4000000953674320
Vector: pJC40 (plasmid)
Promoters: Promoter for expression T7 (phi10)
Construction: pJC20, pET16b
Marker(s):ampR
Construct size (kb): 2.400000095367432
Features: marker(s): ampR
other: Factor Xa cleavage site
other: poly (His) 10
promoter for expression: T7 (phi10)
replicon: pMB1
MCS: NdeI...ApaI
ribosome-binding site: Shine-Dalgarno
terminator: T phi
Applications
encodes removable tag for protein isolation
expression vector
vector for generating nested deletions of cloned inserts
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.4; XbaI--2.4; HindIII/PvuI-- 1.5, 0.8.
High-copy-number expression vector (T7-based) that can be used for preparation of nested deletions.
Also encodes an amino-terminal histidine sequence of 10 residues and a factor Xa cleavage site allowing purification of a recombinant gene product by metal chelate chromatography and removal of the amino terminus.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Clos J, Brandau S. pJC20 and pJC40--two high-copy-number vectors for T7 RNA polymerase-dependent expression of recombinant genes in Escherichia coli. Protein Expr. Purif. 5: 133-137, 1994. PubMed: 8054844

Clos J, et al. Molecular cloning and expression of hexameric Drosophila heat shock factor subject to negative regulation. Cell 63: 1085-1097, 1990. PubMed: 2257625

Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid

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