Size (kb): 5.78
Vector: pXC46 (plasmid)
Promoters: Promoter for expression lambda PL
Construction: pXC24, oligo cassette
Construct size (kb): 5.77
Features: initiation codon: ATG
promoter for expression: lambda PL
replicon: ROP copy number control
restriction site: 3' sites HindIII...BamHI
restriction site: 5' SwaI...NsiI/NdeI sites
ribosome-binding site: synthetic Shine-Dalgarno
spacer sequence: ATTTAA
transcription terminator: partially deleted lambda terminator
coding sequence: 14-3-3
in another host, produces protein
vector useful for cloning PCR products
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; HindIII--5.8.
The vector contains a 0.7 kb bovine cDNA that can be excised using the 5' and 3' cloning sites and be replaced by a gene of interest. The bovine insert may be used as a positive control for expression.
One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloning of PCR products. The vectors differ in cloning sites and in translational enhancer and initiation sequences.
The insert is expressed as a Met-His fusion protein. Sequences cloned into the NsiI/NdeI site are expressed as a Met-His fusion protein, while sequences cloned into the SwaI site can be expressed as an unfused protein (must provide ATG in cloned insert).
Cheng X, Patterson TA. Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res. 20: 4591-4598, 1992. PubMed: 1408761