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pXC33b

86995

One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloing of PCR products.  The vectors differ in cloning sites and in translational enhancer and initiation sequences. The vector contains a 0.7 kb bovine cDNA that can be excised using the 5? and 3? cloning sites and be replaced by a gene of interest.  The bovine insert may be used as a positive control for expression.  The lambda R1 terminator is partially deleleted. The order of the major features of the plasmid is : pMB1 ori ? ampR ? EcoRI ? lambda PL promoter ? translational enhancer ? synthetic SD sequence ? 5? cloning site(s) ? cDNA ? 3? cloning sites.Restriction digests of the vector gave the following results (in kb):   BamHI?5.8 ; EcoRI?5.8 ; HindIII?5.8.                                                     - ATCC staff
Organism
Bos taurus, cow
Clone type
Vector
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Escherichia coli TAP308 containing the plasmid
Storage conditions
2°C to 8°C
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
expression vector
in another host, produces protein
vector useful for cloning PCR products

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; HindIII--5.8.
The vector contains a 0.7 kb bovine cDNA that can be excised using the 5' and 3' cloning sites and be replaced by a gene of interest. The bovine insert may be used as a positive control for expression.
One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloning of PCR products. The vectors differ in cloning sites and in translational enhancer and initiation sequences.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
5.776000022888184
Vector name
pXC33b (plasmid)
Type of vector
plasmid
Construction
pXC24, oligo cassette
Vector information
restriction site: 5' PacI site
spacer sequence: ATTAATTA
Coding sequence
14-3-3
Enhancer
T7 gene 10
initiation codon
ATG
Markers
ampR
Promoters
Expression: lambda PL
Replicon
ROP copy number control; pMB1
Restriction sites
PacI; HindIII; EcoRV; NheI; BamHI
Ribosome binding site
Synthetic Shine-Dalgarno
Terminator
partially deleted lambda terminator
Transcription terminator
partially deleted lambda terminator
Translational enhancer
T7 gene 10

Insert information

Insert size (kb)
0.69999999999999996
Type of DNA
cDNA
Insert source
brain
Insert tissue
brain
Gene product
[14-3-3]

Handling information

History

Depositors
TA Patterson

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Cheng X, Patterson TA. Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res. 20: 4591-4598, 1992. PubMed: 1408761

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