Xenopus laevis NotI/SalI (ATCC® 86985)

Organism: Xenopus laevis, frog, South African clawed  /  Clone Type: Library  /  Depositors: MJ Castanon

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Designations Xenopus laevis NotI/SalI
Species Xenopus laevis, frog, South African clawed
Depositors MJ Castanon
Tissue: oocyte
Insert size range: 1 - 2 kb
Genome Equivalent: 0.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

 Frozen glycerol stock of library in Escherichia coli HB101.

Constructed using a SalI-containing oligo(dT) primer and adaptors to result in 5'NotI and 3'SalI ends.
pMC944 is a YE-type vector permitting expression from the mating factor alpha 1 promoter. Unique XbaI and SphI sites in the promoter and ADH2 terminator, respectively, allow transfer of the expression cassette.
The vector does not contain an initiation codon. It must be provided by the cDNA.
Verified by PCR to contain clones for B4, D7, enolase, Eg1, and nucleoplasmin. The low abundance Vg1 was not detected.

Spevak W, et al. Saccharomyces cerevisiae cdc15 mutants arrested at a late stage in anaphase are rescued by Xenopus cDNAs encoding N-ras or a protein with beta-transducin repeats. Mol. Cell. Biol. 13: 4953-4966, 1993. PubMed: 8393141