Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 43.1
Vector: Charon BS(-) (phage, lambda - replacement)
Promoters: Promoter for in vitro transcription T3
Construction: Charon 15, pBluescript KS M13(-)
Construct size (kb): 43.1
Features: insert detection: lacZ'
other: left arm
other: pBluescript KS -
other: right arm
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
lambda vector allowing conversion to plasmid
vector containing primer sites useful for sequencing
vector for constructing cDNA libraries
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--31.0, 15.5, 6.0; EcoRI--25.0 (doublet); SalI--27.0, 13.5, 9.4; XhoI--27.0, 13.0, 9.2.
Vector for high-efficiency directional cloning. Clones can be converted to plasmids by recircularization after a NotI digest.
Charon BS(-) (ATCC 77446)
and Charon BS(+) (ATCC 77447)
differ in the orientation of the M13 origin of replication.
Swaroop A, Weissman SM. Charon BS(+) and (-), versatile lambda phage vectors for constructing directional cDNA libraries and their efficient transfer to plasmids. Nucleic Acids Res. 16: 8739, 1988. PubMed: 2971160