GL1 (ATCC® 77440)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: A Swaroop

Permits and Restrictions

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Designations GL1
Species Homo sapiens, human
Depositors A Swaroop
Origin
glioblastoma U-138 MG cell line
Vector
Vector : lambda SHK
Intact vector size (kb) : unknown
Vector type : phage
Construction : Charon BS, pSH4
Features (with orientation and position, when available) :
Marker : kanR (KmR)
Replicon : lambda, pMB1
Terminator : SV40 T antigen
Vector
Tissue: glioblastoma U-138 MG cell line
Type of insert: cDNA
Insert size range: > 0.8 kb
Genome Equivalent: 0.0
Genome: human
Vector: Lambda SHK
Vector ends : Modification : 5' EcoRI, 3' HindIII
Number of independent recombinants : 2.1 x 106
Titer : 5.0 x 107 pfu/ml
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Frozen bacteria-free phage lysate.
Comments
Titer determined on E. coli LE392.
cDNA synthesis was primed by oligo-dT, and a synthetic oligonucleotide (5'GCTTGAATTCAAGC 3') was added for directional cloning.
References

Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. Cytogenet. Cell Genet. 64: 292-294, 1993. PubMed: 8404057

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