FB3 (ATCC® 77435)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: A Swaroop

Permits and Restrictions

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Designations FB3
Species Homo sapiens, human
Depositors A Swaroop
Origin
brain, 23 week fetus
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon BS(-)
Intact vector size: 43.100
Type of vector: phage
Vector end: EcoRI
Vector end: HindIII
Cloning sites: EcoRI HindIII
Polylinker sites: NotI BamHI EcoRI EcoRV HindIII SalI XhoI
Cloning capacity: 8.9 kb
Construction: Charon 15, pBluescript KS M13(-)
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
other: left arm, ->, 1-21225
other: pBluescript KS -, ->, 21226-24184
MCS: NotI...KpnI, ->, 21226-21315
promoter for in vitro transcription: T3, <-, 21327-21346
promoter: lac, <-, 21416-21444
replicon: pMB1, 21771-21771
marker(s): ampR, <-, 22529-23389
replicon: f1, ->, 23520-23976
promoter for in vitro transcription: T7, ->, 24140-24159
MCS: SacI...NotI, ->, 24172-24184
other: right arm, ->, 24185-43100
Cross references:
Vector
Tissue: brain, 23 week fetus
DNA: cDNA
Insert size range: > 1.0 kb
Genome Equivalent: 0.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen
Comments
cDNA synthesis was primed by oligo-dT, and a synthetic oligonucleotide (5'GCTTGAATTCAAGC 3') was added for directional cloning.
References

Swaroop A. Construction of directional cDNA libraries from human retinal tissue/cells and their enrichment for specific genes using an efficient subtraction procedure. Methods Neurosci. 15: 285-300, 1993.

Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. Cytogenet. Cell Genet. 64: 292-294, 1993. PubMed: 8404057

Swaroop A, Weissman SM. Charon BS(+) and (-), versatile lambda phage vectors for constructing directional cDNA libraries and their efficient transfer to plasmids. Nucleic Acids Res. 16: 8739, 1988. PubMed: 2971160

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