pUN90 [pNN338] (ATCC® 77269)

Applications: YC-type (centromeric) shuttle vectormutation detectionshuttle vectorvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: SJ Elledge

Permits and Restrictions

View Permits

Designations pUN90 [pNN338]
Depositors SJ Elledge
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.5999999046325680
Vector: pUN90 (plasmid)
Promoters: Promoter lac
Construction: pUC18
Marker(s):ampR,HIS3
Construct size (kb): 5.599999904632568
Features: insert detection: lacZ'
marker(s): HIS3
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: pMB1
MCS: EcoRI...HindIII
centromere: CEN4
Applications
YC-type (centromeric) shuttle vector
mutation detection
shuttle vector
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.6; SacI--5.6; SalI--5.6.
YC-type shuttle vector permitting visual detection of recombinants by lacZalpha complementation.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70: 303-312, 1988. PubMed: 3063604

Related Products
component of:ATCC 87634
component of:ATCC 87637
Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map