Size (kb): 7.8220000267028810
Vector: pFL46L (plasmid)
Construct size (kb): 7.822000026702881
Features: marker(s): ampR, LEU2
replicon: pMB1, 2 micron
YE-type (episomal) shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--7.8; BglII--4.9, 2.9; ClaI--3.7, 2.3, 1.9.
Contrary to published data, this vector does not permit visual detection of recombinants using the assay for alpha complementation.
Shows increased stability because of the presence of STB. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 6.3.
YE-type high copy number shuttle vector.
The order of the major features in this plasmid is: NotI/MCS/HindIII - LEU2 - STB -2 micron ori - pMB1 ori - ampR.
Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470
Struhl K, et al. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc. Natl. Acad. Sci. USA 76: 1035-1039, 1979. PubMed: 375221
Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589
Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730
Francois Lacroute, personal communication