|
Vector Information
|
Size (kb): 6.0630002021789550 Vector: pFL44L (plasmid) Construction: pUC19 Marker(s):URA3,ampR Construct size (kb): 6.063000202178955 Features: marker(s): ampR, URA3
replicon: pMB1, 2 micron
|
|
Applications
|
YE-type (episomal) shuttle vector shuttle vector
|
|
Comments
|
Restriction digests of the clone give the following sizes (kb): BamHI--6.1; BglII--5.0, 1.1; ClaI--3.9, 2.25. Contrary to published data, this vector does not permit visual detection of recombinants using the assay for alpha complementation. Shows increased stability because of the presence of STB. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 4.0. YE-type high copy number shuttle vector. The order of the major features in this plasmid is: NotI/MCS/HindIII - URA3 - STB -2 micron ori - pMB1 ori - ampR.
|
|
References
|
Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470
Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589
Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827
Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730
Francois Lacroute, personal communication
|