Size (kb): 6.0780000686645510
Vector: pFL46S (plasmid)
Promoters: Promoter lac
Construct size (kb): 6.078000068664551
Features: insert detection: lacZ'
marker(s): ampR, LEU2
replicon: pMB1, 2 micron
YC-type (centromeric) shuttle vector
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--6.1; BglII--3.3, 2.8; ClaI--3.7, 1.9, 0.5.
The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 15.5.
YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - LEU2 - 2 micron ori - pMB1 ori - ampR.
Broach JR, et al. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8: 121-133, 1979. PubMed: 395029
Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589
Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730