LL22NL02 (ATCC® 57779)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: P De Jong, JA Garnes

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Designations LL22NL02
Species Homo sapiens, human
Depositors P De Jong, JA Garnes
hybrid J460-51 cell line
Tissue: hybrid J460-51 cell line
Chromosome: 22
DNA: genomic
Digest: Partial
Insert size range: 15 kb average
Genome Equivalent: 15.0
Coverage: Clones for "complete" coverage: 4500.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 ml)
Purity (human clones): 83%. Contaminants (hamster): 3%.
The J640-51 cell line was provided by C. Jones, Eleanor Roosevelt Institute of Cancer Research, Denver, CO 80206. It is described in Cytogenet. Cell Genet. 28: 181-194, 1980 and Somat. Cell. Mol. Genet. 17: 117-136, 1991.
Chromosomes for library construction were isolated by Ger van der Engh using flow-sorting.
Genomic equivalents (average redundancy) were calculated by correcting for purity and assuming 15 kb average inserts and 54 Mb/chromosome 22.

Dunn IS, Blattner FR. Charons 36 to 40: multi enzyme, high capacity, recombination deficient replacement vectors with polylinkers and polystuffers. Nucleic Acids Res. 15: 2677-2698, 1987. PubMed: 3031608

Pieter De Jong, personal communication