LA16NS02 (ATCC® 57730)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: LL Deaven

Permits and Restrictions

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Designations LA16NS02
Species Homo sapiens, human
Depositors LL Deaven
Origin
skin fibroblast, male, HSF7 cell line
Applications
This is preserved in a solution containing 10% glycerol and 0.43 M CsCl.
Vector
Tissue: skin fibroblast, male, HSF7 cell line
Chromosome: 16
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 2.0
Coverage: Clones for "complete" coverage: 16000.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
Of 11 unique sequence clones tested, 1 mapped to chromosome 16.
The library LA16NS02, ATCC 57730, 2.0 genomic equivalents, is distributed together with LA16NS03, ATCC 57749, 21.7 genomic equivalents, because there is a possibility that a sequence in one may not be found in the other.
This is preserved in a solution containing 10% glycerol and 0.43 M CsCl. The CsCl should be diluted to less than 40 mM before infection.
References

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

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