LA07NS01 (ATCC® 57722)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: LL Deaven

Permits and Restrictions
Permits and Restrictions

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Designations LA07NS01
GenBank Definition

H.sapiens CD36 gene exon 1.

H.sapiens CD36 gene exon 2.

GenBank Number

Z32752

Z32753

Species Homo sapiens, human
Depositors LL Deaven
Origin
hamster/human MR3.31 6TG6 hybrid cell line
Applications
This is preserved in a solution containing 10% glycerol and 1.4 M CsCl.
Of 496 probes analyzed after screening for human/hamster repeats, 40% were hamster specific, 39 contained moderately repetitive elements, and 258 (52%) were human-specific single-copy probes.
Vector
Tissue: hamster/human MR3.31 6TG6 hybrid cell line
Chromosome: 7
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 9.199999809265137
Coverage: Clones for "complete" coverage: 26000.0
Biosafety Level 1
Shipping Information Distributed: frozen
Comments
This is preserved in a solution containing 10% glycerol and 1.4 M CsCl. The CsCl should be diluted to less than 40 mM before infection.
About 26% hamster impurity by in situ hybridization of sorted chromosomes; about 48% hamster impurity by plaque hybridization.
Hamster impurity is 20-30%.
Of 496 probes analyzed after screening for human/hamster repeats, 40% were hamster specific, 39 contained moderately repetitive elements, and 258 (52%) were human-specific single-copy probes. The size range of these was 0.5 - 6.5 kb.
Of 55 unique sequence clones, 53 map to chromosome 7.
Of 19 unique sequence clones, 18 map to chromosome 7.
This is the second amplification of this library.
References

Rommens JM, et al. Identification and regional localization of DNA markers on chromosome 7 for the cloning of the cystic fibrosis gene. Am. J. Hum. Genet. 43: 645-663, 1988. PubMed: 2903665

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Product Sheet
Product Sheet
Product Sheet