LL21NS02 (ATCC® 57713)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: M Van Dilla, JA Garnes

Permits and Restrictions
Permits and Restrictions

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Designations LL21NS02
Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
Origin
skin fibroblast 811 cell line
Vector
Tissue: skin fibroblast 811 cell line
Chromosome: 21
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 20.0
Coverage: Clones for "complete" coverage: 7800.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
The #21 peak was very well-isolated; little debris. The background impurity in the sort window: 30%.
The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 60.
References

. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.

Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744

Marvin Van Dilla, personal communication

Product Sheet
Product Sheet
Product Sheet