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Escherichia coli (Migula) Castellani and Chalmers

47076

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Escherichia coli strain MG165 is a host strain with well-characterized restriction maps for NotI, SfiI, XbaI, and BlnI. This bacterial strain can be used as a molecular size marker for pulsed-field gel electrophoresis.
Product category
Bacteria
Classification
Enterobacteriaceae, Escherichia
Strain designation
MG1655
Type strain
No
Genome sequenced strain
Yes
Applications
Molecular biology
Food production research
Product format
Frozen
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Transformation host
Bacteriophage host

Potential strain for single cell protein production for food or feed

Preceptrol
No

Characteristics

Genotype
F- lambda-
Comments
Electronic access to the correlations between the restriction map and the ordered phage library of Kohara are available over the Internet by anonymous ftp at these hosts: ncbi.nlm.nih.gov and utmmg.med.uth.tmc.edu.
Host strain with well-characterized restriction maps for NotI, SfiI, XbaI and BlnI. Can be used as a molecular size marker for pulsed-field gel electrophoresis.
AE000111 through AE000510 are individual GenBank numbers for the complete sequence, U00096
Derived from the parent strain W1485 by curing of the F plasmid.
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Medium
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
  1. Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
  2. Aseptically transfer the entire contents to a 5-6 mL tube of #1065 broth. Additional test tubes can be inoculated by transferring 0.1 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #1065 plate and/or #1065 agar slant.
  4. Incubate at 37°C for 24 hours.
Handling notes
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Escherichia coli (Migula) Castellani and Chalmers
Depositors
GM Weinstock, University of Texas Medical School
Chain of custody
ATCC <-- GM Weinstock <-- MG1655
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Cross references
GenBank T03427
GenBank unknown
GenBank U00096 complete sequence
GenBank X72920 intercistronic region
GenBank X64592 gene for ribosomal protein S7, partial
GenBank L10328 chromosomal region from 81.5 to 84.5 minutes
GenBank L19201 chromosomal region from 87.2 to 89.2 minutes
GenBank M87049 chromosomal region from 84.5 to 86.5 minutes
GenBank U00006 chromosomal region from 89.2 to 92.8 minutes
GenBank X54797 firA gene, firA(200) and firA(201) allele, and skp gene (3' end)
GenBank M62044
GenBank EST00100
GenBank M62192
GenBank EST00253
GenBank M62197
GenBank EST00258
GenBank M62210
GenBank EST00272
GenBank M77859
GenBank EST01443
GenBank M77882
GenBank EST01466
GenBank M77976
GenBank EST01560
GenBank M78033
GenBank EST01620
GenBank M78047
GenBank EST01634
GenBank M78056
GenBank EST01643
GenBank M78079
GenBank EST01667
GenBank M78086
GenBank EST01676
GenBank M78107
GenBank EST01697
GenBank M78159
GenBank EST01751
GenBank M78227
GenBank EST01824
GenBank M78254
GenBank EST00402
GenBank M78321
GenBank EST00469
GenBank M78347
GenBank EST00495
GenBank M78405
GenBank EST00553
GenBank M78451
GenBank EST00599
GenBank M78473
GenBank EST00621
GenBank M78478
GenBank EST00626
GenBank M78683
GenBank EST00831
GenBank M78693
GenBank EST00841
GenBank M78724
GenBank EST00872
GenBank M78737
GenBank EST00885
GenBank M78741
GenBank EST00889
GenBank M78742
GenBank EST00890
GenBank M78743
GenBank EST00891
GenBank M78744
GenBank EST00892
GenBank M78749
GenBank EST00897
GenBank M78762
GenBank EST00910
GenBank M78765
GenBank EST00913
GenBank M78769
GenBank EST00917
GenBank M78771
GenBank EST00919
GenBank M78774
GenBank EST00922
GenBank M78779
GenBank EST00927
GenBank M79070
GenBank EST01218
GenBank M85331
GenBank EST01840
GenBank M85388
GenBank EST01902
GenBank M85391
GenBank EST01905
GenBank M85428
GenBank EST01944
GenBank M85433
GenBank EST01949
GenBank M85453
GenBank EST01969
GenBank M85458
GenBank EST01974
GenBank M85459
GenBank EST01975
GenBank M85463
GenBank EST01979
GenBank M85467
GenBank EST01983
GenBank M85487
GenBank EST02003
GenBank M85489
GenBank EST02005
GenBank M85491
GenBank EST02007
GenBank M85500
GenBank EST02016
GenBank M85512
GenBank EST02028
GenBank M85535
GenBank EST02051
GenBank M85652
GenBank EST02169
GenBank M85838
GenBank EST02362

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Disclosures
This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Blattner FR. Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res. 21: 5408-5417, 1993. PubMed: 8265357

Blattner FR, et al. The complete genome sequence of Escherichia coli K-12. Science 277: 1453-1462, 1997. PubMed: 9278503

Burland V, et al. DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication. Genomics 16: 551-561, 1993. PubMed: 7686882

Daniels DL, et al. Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes. Science 257: 771-778, 1992. PubMed: 1379743

Dicker IB. Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli. J. Bacteriol. 173: 334-344, 1991. PubMed: 1987124

View All Curated Citations for this Product

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