Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47017)

Strain Designations: DB1257  /  Type Strain: no  /  Biosafety Level: 1

Classification Enterobacteriaceae, Escherichia
Strain Designations DB1257
Application
transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Preceptrol® no
Genotype F- delta(gpt-proA)62 thr-34::Tn10 trp-89::Tn5 supF58 lacY1 ara-14 galK2 xyl-5 mtl-1 leuB6 hisG4 argE3 hsdR rac- sbcB15 recB21 recC22 rpsL31 rfbD1 kdgK51 thi-1 tsx-33 lambda-
Type Strain no
Comments
Propagates lambda clones containing inverted repeat sequences.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Genotype F- delta(gpt-proA)62 thr-34::Tn10 trp-89::Tn5 supF58 lacY1 ara-14 galK2 xyl-5 mtl-1 leuB6 hisG4 argE3 hsdR rac- sbcB15 recB21 recC22 rpsL31 rfbD1 kdgK51 thi-1 tsx-33 lambda-
Name of Depositor AR Wyman
References

Donlon TA, et al. Isolation of molecular probes associated with the chromosome 15 instability in the Prader-Willi syndrome. Proc. Natl. Acad. Sci. USA 83: 4408-4412, 1986. PubMed: 3012567

Wyman AR, et al. Propagation of some human DNA sequences in bacteriophage lambda vectors requires mutant Escherichia coli hosts. Proc. Natl. Acad. Sci. USA 82: 2880-2884, 1985. PubMed: 3157994

Wyman AR, et al. Factors which equalize the representation of genome segments in recombinant libraries. Gene 49: 263-271, 1986. PubMed: 2952554

Ruther U, et al. pUR222, a vector for cloning and rapid chemical sequencing of DNA. Nucleic Acids Res. 9: 4087-4098, 1981. PubMed: 6272193