Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47000)

Strain Designations: NM522 [71-18 hsddelta5]  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations NM522 [71-18 hsddelta5]
Application
transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Shipping Information

Shipped:  Freeze dried E. coli

Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype F' lacIq delta(lacZ)M15 proA+ proB+ delta(hsdMS-mcrB)5 delta(lac-proAB) supE thi-1 lambda-
Type Strain no
Comments
A lacZ host lacking a restriction system for M13 and other filamentous bacteriophage.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Genotype F' lacIq delta(lacZ)M15 proA+ proB+ delta(hsdMS-mcrB)5 delta(lac-proAB) supE thi-1 lambda-
Name of Depositor D Mead
References

Gough JA, Murray NE. Sequence diversity among related genes for recognition of specific targets in DNA molecules. J. Mol. Biol. 166: 1-19, 1983. PubMed: 6304321

Mead DA, et al. Single stranded DNA SP6 promoter plasmids for engineering mutant RNAs and proteins: synthesis of a 'stretched' preproparathyroid hormone. Nucleic Acids Res. 13: 1103-1118, 1985. PubMed: 3858795

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation