Saccharomyces cerevisiae Meyen ex E.C. Hansen (ATCC® 200881)

Alternate State: Candida robusta Diddens et Lodder  /  Strain Designations: BY4718  /  Product Format: frozen

Deposited As Saccharomyces cerevisiae Hansen, teleomorph
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain Designations BY4718
Alternate State Candida robusta Diddens et Lodder
Application
transformation host with non-revertable mutations for auxotrophic markers
transformation host with non-revertable designer deletions
Biosafety Level 1
Product Format frozen
Type Strain no
Genotype MATa met15delta0 ura3delta0 RefBrachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801
Preceptrol® no
Mating Type a
Comments
One of a series of strains (ATCC 200866-200902) useful for gene disruption experiments.
The strains differ in mating type and in non-revertable mutations for one or more of these auxotrophic markers: ade2delta::hisG, his3delta200, leu2delta0, lys2delta0, met15delta0, trp1delta63, ura3delta0.
Morphology After 3 days on YEPD medium at 25°C, colonies are off-white, smooth, butyrous. Cells are ovoid or irregular; single, in pairs or small clusters.
Medium ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
Growth Conditions Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
No DNA sequencing was performed in house on this product.
Morphology After 3 days on YEPD medium at 25°C, colonies are off-white, smooth, butyrous. Cells are ovoid or irregular; single, in pairs or small clusters.
Name of Depositor JD Boeke
Special Collection NCRR Contract
References

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801