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Quantitative Synthetic SARS-CoV-2 RNA: ORF, E, N (ATCC® VR-3276SD)

Product Format: frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 µL per vial with Biomatrica RNAstable

Permits and Restrictions

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Agent Quantitative Synthetic SARS-CoV-2 RNA: ORF, E, N
Applications

VR-3276SD™ has replaced VR-3276T™ in our catalog.

  • VR-3276SD™ is identical to VR-3276T™ in construction and sequence.
  • VR-3276SD™ differs from VR-3276T™ only in the operational designation.  Whereas VR-3276T™ is an ISO 9001 manufactured product, VR-3276SD™ is manufactured under ISO 13485 guidelines and is therefore suitable for a broader range of usage.


ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 µL per vial with Biomatrica RNAstable
Storage Conditions -70°C or colder
Intended Use The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.

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Comments

VR-3276SD™ has replaced VR-3276T™ in our catalog.

  • VR-3276SD™ is identical to VR-3276T™ in construction and sequence.
  • VR-3276SD™ differs from VR-3276T™ only in the operational designation.  Whereas VR-3276T™ is an ISO 9001 manufactured product, VR-3276SD™ is manufactured under ISO 13485 guidelines and is therefore suitable for a broader range of usage.

Manufactured under ISO 13485 guidance

Preparation includes fragments from ORF 1ab, Envelope, and Nucleocapsid regions.

The following primers and probe can be used with this nucleic acid preparation.
Forward primer (5’ to 3’): GACCCCAAAATCAGCGAAAT
Reverse primer (5’ to 3’): TCTGGTTACTGCCAGTTGAATCTG
Probe (5’ to 3’): FAM/ACCCCGCATTACGTTTGGTGGACC/BHQ1

Name of Depositor ATCC
References

Corman VM, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.  Euro Surveill 25(3): doi: 10.2807/1560-7917.ES.2020.25.3.2000045, 2020. PubMed: 31992387

Research Use Only 2019 – Novel Coronavirus (2019-nCoV) Real-time RT-PCR Primer and Probe Information. https://www.cdc.gov/coronavirus/2019-ncov.  Centers for Disease Control and Prevention.

Specific primers and probes for detection of 2019 novel coronavirus.  http://ivdc.chinacdc.cn. Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention.

Diagnostic detection of Novel coronavirus 2019 by Real time RT-PCR. https://www.who.int/ Department of Medical Sciences, Ministry of Public Health, Thailand.

Detection of 2019 novel coronavirus (2019-nCov) in suspected human cases by RT-PCR. https://www.who.int/ School of Public Health, The University of Hong Kong.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
FAQs
  1. VR-3276SD assays


    Date Updated: 4/13/2020