Rabbit polyclonal antibody prepared against the spike glycoprotein of Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2, Wuhan-Hu-1) was ammonium sulfate precipitated from pooled rabbit serum and purified by protein G affinity chromatography.
Molecular weight for heavy and light chains were evaluated using Agilent analysis. Lane 1: Molecular weight marker (kDa). Lane 2: ATCC VR-1997.
Western blot analysis was performed using 0.5 μg SARS-CoV-2 S glycoprotein ACROBiosystems), 1:15,000 dilution of ATCC VR-1997, and 1:10,000 dilution of anti-rabbit IRDye® conjugate (LI-COR Biosciences). Lane 1: MagicMark™ XP Protein Standard (Thermo Fisher Scientific). Lane 2: SARS-CoV-2 S Glycoprotein.
Indirect ELISA was performed using 1 μg/mL SARS-CoV-2 S glycoprotein (ACROBiosystems), 1:250 to 1:512,000 dilution of ATCC VR-1997, and 1:10,000 dilution of anti-rabbit HRP conjugate. The polyclonal antibody was also reactive to Human coronavirus HKU1 S glycoprotein.
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