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Polyclonal Anti-SARS-CoV-2 Spike Glycoprotein (ATCC® VR-1997)

Product Format: frozen 100 µL per vial

Agent Polyclonal Anti-SARS-CoV-2 Spike Glycoprotein
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 100 µL per vial
Storage Conditions -20°C or colder
Comments

Rabbit polyclonal antibody prepared against the spike glycoprotein of Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2, Wuhan-Hu-1) was ammonium sulfate precipitated from pooled rabbit serum and purified by protein G affinity chromatography.

Western Blot Analysis

ELISA

Molecular weight for heavy and light chains were evaluated using Agilent analysis. Lane 1: Molecular weight marker (kDa). Lane 2: ATCC VR-1997.

Western blot analysis was performed using 0.5 μg SARS-CoV-2 S glycoprotein ACROBiosystems), 1:15,000 dilution of ATCC VR-1997, and 1:10,000 dilution of anti-rabbit IRDye® conjugate (LI-COR Biosciences). Lane 1: MagicMark™ XP Protein Standard (Thermo Fisher Scientific). Lane 2: SARS-CoV-2 S Glycoprotein.

Indirect ELISA was performed using 1 μg/mL SARS-CoV-2 S glycoprotein (ACROBiosystems), 1:250 to 1:512,000 dilution of ATCC VR-1997, and 1:10,000 dilution of anti-rabbit HRP conjugate. The polyclonal antibody was also reactive to Human coronavirus HKU1 S glycoprotein.

IRDye® is a registered trademark of LI-COR Biosciences. MagicMark™ XP is a trademark of Thermo Fisher Scientific. Agilent® is a registered trademark of Agilent Technologies, Inc.

Name of Depositor ATCC
References

Wu F, et al. A New Coronavirus Associated with Human Respiratory Disease from China. Nature 579: 265-269, 2020. PubMed: 32015508