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1.800.638.6597

H21F8-1 (ATCC^® CRL-8018^™)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) / Cell Type: hybridoma: B lymphocyte / Disease: hepatitis

H21F8-1 ATCC^® CRL-8018^™

frozen

For-Profit: $551.00 Non-Profit: $468.35

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Permits and Restrictions	View Permits
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	hepatitis
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation	Spleen cells were fused with P3-NSI/1Ag4-1 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against hepatitis B virus surface antigen (HBsAg)
Cellular Products	immunoglobulin; monoclonal antibody; against hepatitis B virus surface antigen (HBsAg)
Comments	Animals were immunized with hepatitis B virus surface antigen. Spleen cells were fused with P3-NSI/1Ag4-1 myeloma cells.

Complete Growth Medium	Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Isotype	IgM

Name of Depositor	Massachusetts General Hospital
Deposited As	mouse (B cell); mouse (myeloma)
U.S. Patent Number	4,271,145
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
References	Wands JR, Zurawski VR Jr.. Process for producing antibodies to hepatitis virus and cell lines therefor. US Patent 4,271,145 dated Jun 2 1981

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
References	Wands JR, Zurawski VR Jr.. Process for producing antibodies to hepatitis virus and cell lines therefor. US Patent 4,271,145 dated Jun 2 1981