UACC-2087 (ATCC® CRL-3180)

Organism: Homo sapiens, human  /  Disease: adenocarcinoma

Organism Homo sapiens, human
Product Format frozen
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 51 years old
Gender female
Applications
The cells have been used in studies of p53 mutation, as well as the relegation of p53 on DSC3 and MAPSIN.
Storage Conditions liquid nitrogen vapor phase
Karyotype range=48-67; modal number=60-64
Images
Receptor Expression
epidermal growth gactor receptor (EGFR), expressed
neu (ErbB2; HER2), not expressed
estrogen receptor (ER), not expressed
progesterone receptor (PR), not expressed
Comments
In addition, p53 V216M mutation was found in this cell line. The cells have been used in studies of p53 mutation, as well as the relegation of p53 on DSC3 and MAPSIN
UACC-2087 was derived from a 51 year-old female with breast cancer. The patient underwent chemotherapy. This cell line is a triple negative breast cancer cell line. Estrogen receptors, progesterone receptors and HER-2/neu are negative in the cells. The expression of tumor suppressor MAPSIN and cell adhesion molecules DSC3 is also negative in this cell line.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 5%
  • 0.01 mg/ml transferrin (final conc.)
  • 0.01 mg/ml insulin (final conc.)
  • 5 µg/ml (55 U/ml) catalase (final conc.)
  • 3.6 µg/ml (0.01 mM) hydrocortisone (final conc.)
  • extra 2 mM glutamine

Subculturing
Note: This cell line grows slowly.
Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37.0°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 3 x 104 to 4 x 104 viable cells/cm2 is recommended.
  7. Incubate cultures at 37.0°C. Subculture when the cell concentration is between 7 X 104 to 9 X 104 cells/cm2.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
Medium renewal: every 3 to 4 days
Cryopreservation
Freeze medium: complete growth medium, 80%; FBS, 10%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 100%
Name of Depositor K Brown
References

Rice JC, et al. Aberrant methylation of the BRCA1 CpG island promoter is associated with decreased BRCA1 mRNA in sporadic breast cancer cells. Oncogene 17(14): 1807-1812, 1998. PubMed: 9778046

Watts GS, et al. The acetyltransferase p300/CBP-associated factor is a p53 target gene in breast tumor cells. Neoplasia 6(3): 187-194, 2004. PubMed: 15153330

Domann FE, et al. Epigenetic silencing of maspin gene expression in human breast cancers. Int. J. Cancer. 85(6): 805-810, 2000 PubMed: 10709100

Oshiro MM, et al. Mutant p53 and aberrant cytosine methylation cooperate to silence gene expression. Oncogene 22(23): 3624-3634, 2003. PubMed: 12789271

Futscher BW, et al. Chapter 6: Epigenetic Dysregulation of Maspin (SerpinB5) in Cancer Invasion and Metastasis. DNA Methylation, Epigenetics and Metastasis. Cancer Metastasis - Biology and Treatment, 7: 133-155, 2005.

Oshiro MM, et al. Epigenetic silencing of DSC3 is a common event in human breast cancer. Breast Cancer Res. 7(5): R669-R680, 2005. PubMed: 16168112

Futscher BW, et al. Chapter 7: Epigenetic Regulation of Genes That Affect Tumor Cell Adhesion. Cell Adhesion and Cytoskeletal Molecules in Metastasis. Cancer Metastasis - Biology and Treatment, 9: 123-140, 2006.

Robey IF, et al. Regulation of the warburg effect in early-passage breast cancer cells. Neoplasia 10(8): 745-756, 2008. PubMed: 18670636

Basic Documentation
Other Documentation
References

Rice JC, et al. Aberrant methylation of the BRCA1 CpG island promoter is associated with decreased BRCA1 mRNA in sporadic breast cancer cells. Oncogene 17(14): 1807-1812, 1998. PubMed: 9778046

Watts GS, et al. The acetyltransferase p300/CBP-associated factor is a p53 target gene in breast tumor cells. Neoplasia 6(3): 187-194, 2004. PubMed: 15153330

Domann FE, et al. Epigenetic silencing of maspin gene expression in human breast cancers. Int. J. Cancer. 85(6): 805-810, 2000 PubMed: 10709100

Oshiro MM, et al. Mutant p53 and aberrant cytosine methylation cooperate to silence gene expression. Oncogene 22(23): 3624-3634, 2003. PubMed: 12789271

Futscher BW, et al. Chapter 6: Epigenetic Dysregulation of Maspin (SerpinB5) in Cancer Invasion and Metastasis. DNA Methylation, Epigenetics and Metastasis. Cancer Metastasis - Biology and Treatment, 7: 133-155, 2005.

Oshiro MM, et al. Epigenetic silencing of DSC3 is a common event in human breast cancer. Breast Cancer Res. 7(5): R669-R680, 2005. PubMed: 16168112

Futscher BW, et al. Chapter 7: Epigenetic Regulation of Genes That Affect Tumor Cell Adhesion. Cell Adhesion and Cytoskeletal Molecules in Metastasis. Cancer Metastasis - Biology and Treatment, 9: 123-140, 2006.

Robey IF, et al. Regulation of the warburg effect in early-passage breast cancer cells. Neoplasia 10(8): 745-756, 2008. PubMed: 18670636