Bovine herpesvirus 1 (ATCC® VR-864)

Classification: Herpesviridae, Varicellovirus  /  Product Format: frozen

Classification Herpesviridae, Varicellovirus
Deposited As Infectious bovine rhinotracheitis
Agent Bovine herpesvirus 1
Strain Colorado-1 [Cooper 1]
Common Name Infectious bovine rhinotracheitis virus, Bovine rhinotracheitis virus
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Comments
To prevent possible introduction of antibodies and BVDV, use of horse serum is recommended
Effect on Host
CPE, rounding, clumping, and sloughing.
Recommended Host
BT cells (ATCC® CRL-1390™™)
Growth Conditions

Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).

Incubation: 1 to 5 days at 37°C, a 5% CO2 in air atmosphere is recommended.

Effect on Host
CPE, rounding, clumping, and sloughing.
Name of Depositor JJ England
Source Lung of bovine with upper respiratory infection, United States
References

Whitbeck JC, et al. Synthesis, processing, and oligomerization of bovine herpesvirus 1 gE and gI membrane proteins. J. Virol. 70: 7878-7884, 1996. PubMed: 8892910

York CJ, et al. Isolation and identification of infectious bovine rhinotracheitis virus in tissue culture. Proc. Soc. Exp. Biol. Med. 94: 740-744, 1957. PubMed: 13431940