CCD-112CoN (ATCC® CRL-1541)

Organism: Homo sapiens, human  /  Tissue: colon  /  Disease: normal

Organism Homo sapiens, human
Tissue colon
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 22 weeks gestation
Gender female
Karyotype 46, XX; modal number = 46; polyploidy at 5.5%; inv(9)(p13q13)
Clinical Data
female
Comments
The line begins to senesce at population doubling level (PDL) = 76.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,13
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,9
THO1: 6,7
TPOX: 8
vWA: 16,18
Population Doubling Capacity The line begins to senesce at population doubling level (PDL) = 76.
Name of Depositor ATCC