MC-SV-HUC T-2 (ATCC® CRL-9519)

Organism: Homo sapiens, human  /  Tissue: ureter  / 

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue ureter
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 11 years
Gender male
Applications
The line has been repeatedly tested for production of infectious SV40 using an African Green Monkey kidney cell plaque assay, and has always tested negative.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Clinical Data
male
Genes Expressed
uroepithelial keratins
Cellular Products
uroepithelial keratins
Tumorigenic Yes
Effects
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Comments
The cells were initially transformed with SV40 virus, and later treated with 3-methylcholanthrene (MCA).
While the parental line (SV-HUC-1, see ATCC CRL-9520) was not tumorigenic and had a balanced chromosome composition, the MCA treated line became tumorigenic and aneuploid.
The line has been repeatedly tested for production of infectious SV40 using an African Green Monkey kidney cell plaque assay, and has always tested negative.
Stress (such as chemical exposure) could possibly activate the virus.
Complete Growth Medium Ham's F12 medium, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor Wisconsin Alumni Res. Fndn.
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
References

Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990