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Human coronavirus 229E (ATCC® VR-740)

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Classification: Coronaviridae, Coronavirus  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

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Classification Coronaviridae, Coronavirus
Agent Human coronavirus 229E
Strain 229E
Common Name Human coronavirus
Verified By Whole-genome Sequencing
No hemolysis production.
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Possibility of cross-reactions with other coronaviruses not thoroughly characterized at time of deposit (1973); does not cross-react with human myxoviruses. Ether sensitive. No hemolysis production. Unstable at 24 & 37C; relatively stable at 10C.
Harvest 26-50 hrs post adsorption before CPE is visible. Does not agglutinate chicken, guinea pig, human O, sheep, mouse or rat RBC. Size 89Mu (gradocol filtration). [RU1 are human embryonic lung fibroblasts developed by CDC]
Effect on Host
CPE, rounding and sloughing. Starts as individual cell rounding that spreads, but over time uninfected cells repopulate monolayer.
Recommended Host
MRC-5 (ATCC® CCL-171™)
Growth Conditions Temperature: 35°C
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Incubation: 2-7 days, a 5% CO2 in air atmosphere is recommended.
Effect on Host
CPE, rounding and sloughing. Starts as individual cell rounding that spreads, but over time uninfected cells repopulate monolayer.
Name of Depositor HS Kaye
Special Collection ATCC
Source Nasal and throat swabs from man with mild upper respiratory illness

Hamre D, Procknow JJ. A new virus isolated from the human respiratory tract. Proc. Soc. Exp. Biol. Med. 121: 190-193, 1966. PubMed: 4285768

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019