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The optimal pH for cell culture medium
What is the optimal pH for cell culture medium?

The optimal pH for most cell lines is 7.4. Most medium contains phenol red as an indicator- it changes color in response to pH. If the phenol red indicator in the medium is dark pink or purple, the pH of the medium is basic. If the phenol red indicator in the medium is orange or yellow, the pH of the medium is acidic. The pH of the medium should be measured after preparing medium from powder or adding supplements to liquid.
Date Created07/17/2012 07:10 PM
Date Updated03/27/2014 08:41 PM

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