Your trusted resource for cell lines, microbial strains, biological reagents, and innovative solutions invites you to view one of the exciting webinars in our Excellence in Research series. Each webinar featured a highly experienced ATCC Scientist who demonstrated how to utilize ATCC products and services to advance your research. The webinars were broadcasted live, ran 30 to 45 minutes, and were followed by a 10 minute question and answer period.
2016 Excellence in Research Series
Discovering ATCC Primary Immune Cells: Model Systems to Study the Immune and Cardiovascular Systems
James Clinton, Ph.D., Scientist, ATCC
Recorded July 14, 2016
Abstract: Hematopoietic stem cells (HSCs) are capable of differentiating into the formed elements of blood: leukocytes, erythrocytes, and thrombocytes. While these distinct cell types display specific immune, transport, and hemostatic phenotypic functions, they are all derived from HSCs. In this webinar we will present data showing that ATCC marrow- and blood-derived primary immune cells, such as bone marrow and cord blood CD34+ cells as well as peripheral blood CD14+ monocytes can be induced to differentiate into various hematopoietic lineages. This webinar will also highlight the use of peripheral blood mononuclear cells to investigate the immunosuppressive capabilities of primary and hTERT-immortalized mesenchymal stem cells in a T-cell proliferation assay. Download Presentation (912 KB.pdf) View Recording.
- HSCs are a rapidly advancing area of research producing insights into cellular plasticity, immune function, and disease.
- Cryopreserved, purified blood cells can be cultured in vitro and induced to differentiate into downstream lineages or used for functional assays.
- ATCC offers a variety of blood cell type from multiple tissues that are useful in many applications and research areas.
Biography: James Clinton, Ph.D., works in new product development, with a focus on primary cells and advanced, physiological relevant culture systems using novel technologies. Previously he worked at University of California, San Diego and the La Jolla Institute for Molecular Medicine. Dr. Clinton attended Washington State University and University of California, San Diego where he studied Neuroscience.
Functionally Characterized Human PBMCs: An Improved In Vitro Model of Human Immune Response
Alexei Miagkov, Ph.D., Senior Scientist, Immunology, ATCC
Recorded June 30, 2016
Abstract: Human Peripheral Blood Mononuclear Cells (PBMCs) contain many of the functional cell types of immune system, and are an ideal model to study the human immune response in vitro. Currently, PBMCs are being used in basic and clinical research areas including basic immunology and cell biology, infectious diseases, vaccine development, tumor immunology, and drug discovery. PBMC are also widely used for monitoring disease progression, designing personalized approaches to treatment, and predicting the occurrence of treatment-associated adverse events. The majority of PBMC applications rely on highly complex cell-based assays; however, phenotypic and functional variability of human PBMCs makes the development and validation of these assays difficult. Additionally, variations in cell collection and cryopreservation protocols can further affect PBMC properties. In this presentation, we demonstrate that pre-screening characterization of lot-specific functional activity of PBMCs is extremely useful for selecting cells to address individual experimental goals. Download Presentation (912 KB.pdf) View Recording.
- PBMCs play a critical role in modern biomedical research; however, the functional activity of these cells is highly variable
- Many factors affecting PBMC variability, including genetic diversity and environmental pressure, are outside of researchers’ control
- Conducting pre-screening and establishing PBMC functional activity profiles offers an attractive solution to address PBMC variability
Biography: Alexei Miagkov leads the Tumor Immunology group at ATCC Cell Systems. Prior to joining ATCC, Dr. Miagkov served as the Scientific Program Manager at PerkinElmer, where he was responsible for the design, validation, and implementation of assays intended for preclinical studies in oncology and inflammatory diseases. He also held progressing scientific roles at Caliper Life Sciences and NovaScreen Biosciences. Dr. Miagkov completed a post-doctoral fellowship in neuro-immunology at Johns Hopkins University, and earned his Ph.D. in cell biology and M.S. in cellular immunology and microbiology from Moscow State University.
Carbapenem-resistant Enterobacteriaceae (CRE) – A Growing Superbug Population
Cara Wilder, Ph.D., Technical Writer, ATCC
Recorded May 5, 2016
Abstract: The discovery of antibiotics in the early twentieth century has revolutionized the treatment of infectious diseases, saving millions of lives and easing the suffering of many. However, as the structure and function of antibiotics have evolved through the efforts of biotech and pharma companies, prokaryotic species are evolving in parallel, fashioning novel and effective methods to avoid therapeutic killing. In the last several decades, this concern has become more pronounced with the emergence of multidrug-resistant organisms in both community- and hospital-acquired infections, resulting in increased morbidity, mortality, and health-care expense. In this presentation, we will discuss the emergence of multidrug-resistant infections with a particular emphasis on the emergence and global spread of carbapenem-resistant Enterobacteraeae strains. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.
- Multidrug-resistant strains are an emerging problem throughout the world
- ATCC acquires, authenticates, and distributes clinically relevant multidrug-resistant strains that are essential to the scientific community
- KPC, NDM, and OXA strains are now available at ATCC
Biography: Dr. Wilder is the Technical Writer for ATCC Microbial Systems. She has a Ph.D. in Microbiology with background experience working with several pathogenic bacterial species in both in vitro and in vivo environments. Dr. Wilder is the author of numerous publications on varying topics of scientific relevance, including quality control, microbial contamination, assay development, proficiency testing, and multidrug resistance.
The ATCC Story: A Ninety Year Celebration
Frank Simione, M.S., Director, Standards, Standards Resource Center, ATCC
Recorded April 28, 2016
Abstract: ATCC celebrated its 90th anniversary in 2015 and in recognition of that milestone this presentation tells the remarkable story of ATCC’s evolution as an organization and its contribution to developments in life sciences for nearly a century. Established in 1925 as the American Type Culture Collection by scientists for scientists, it has become an international resource for life science research and development. Dependent on financial subsidies for more than 75 years, ATCC has since achieved independence as a fully self-sustaining non-profit organization. ATCC is now investing in research and development, and in 2012 was able to establish and fully fund the Global Biological Standards Institute (GBSI).This is the story of that amazing transformation and the role of ATCC in the evolution of the life sciences. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.
- ATCC was founded by scientists for scientists; it is a science-based organization
- ATCC is now fully self-sustainable after dependence on government subsidies and private financial support for more than 75 years
- ATCC contributes to global science and health initiatives by providing biological resources and related services
Biography: Mr. Simione is currently advisor to the ATCC President and CEO, and Director of Standards for the ATCC Standards Resource Center. He has been with ATCC for 41 years and was a member of the senior management team for 26 years. His previous management roles at ATCC have included oversight of operations, government contracts, compliance and safety, patent deposits, workshops and training, biocollections, and standards. Part of his current activities includes documenting ATCC’s 90 year history.
Best Practices in Cryopreservation
Steven Budd, M.S., M.B.A., Product Line Business Specialist, ATCC
Recorded April 21, 2016
Abstract: Cryopreservation is the use of very low temperatures to structurally preserve intact living cells and tissues. Normally, the freezing of water in cells causes catastrophic damage to cellular structure by physical damage of ice formation and increased imbalance of solutes. Cryopreserving cells with the proper cryoprotectants and techniques will maximize viability of cells for cell culture. This webinar presentation will discuss best practices for cryopreservation focusing on determining optimal freezing rates and cryoprotectants, selecting proper containment units, managing a biorepository, and handling cells post-thaw. Emphasis will be placed on reviewing time-proven techniques while introducing newer innovative approaches to maximize reliability in cryopreservation for modern day cell culture.
- For the most accurate administration of DMSO, use Serum Free Cell Freezing Media already formulated with exactly 10% DMSO
- A controlled cooling rate (1 – 3°C /min) is necessary to maximize cell viability when freezing down cell lines
- The CoolCell® is a reliable and consistent cryo-container
Biography: Steven Budd is a Product Line Business Specialist managing cell culture reagents at ATCC. He has 4 years of experience in the product management of scientific tools. Before that, he gained 4 years of experience in biomedical research and cell culture as a research specialist at the University of North Carolina at Chapel Hill. Mr. Budd has a Master’s Degree in Biology from the University of North Carolina at Wilmington and an MBA from North Carolina State University. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.
A Tale of 3 Mummies: A Microbiome Analysis of Life in the Peruvian Andes 1,000 Years Ago
Raul Cano, Ph.D., Director, Microbiome Research, ATCC-CTM and Professor Emeritus, California Polytechnic State University
Recorded April 14, 2016
Abstract: The natural mummification process is a rare and unique process resulting from low temperatures and oxygen levels, and dry weather conditions. In the present study, we characterized the gut microbiome of three pre-Columbian Andean mummies using 16S rRNA gene high-throughput sequencing and metagenomics to understand the preservation and evolution of commensal and pathogenic microorganisms, antimicrobial resistance genes, diet, and the metabolic processes during the natural mummification of the human gut.
- 16S rRNA gene high-throughput sequencing and metagenomics can be used to understand changes to the human microbiome
- Evaluation of microbial populations from mummified remains can provide valuable insight into the medical and cultural aspects of ancient people
- Ancient DNA must be suitable for investigation and should be evaluated through damage analysis studies
- Quality control of the metagenome analysis process is key to ALL studies. It is particularly important in ancient DNA studies to ensure that the results are reflective of the actual sample, not an artifact of DNA extraction, PCR, sequencing, and/or analysis
Biography: Raul Cano, Ph.D., is a Professor Emeritus at California Polytechnic State University and the Director, Microbiome Research at the ATCC Center for Translational Microbiology (ATCC-CTM). He has extensive experience in the fields of microbial ecology, bioremediation, metagenomics, clinical microbiology, and microbiome research. Dr. Cano was named a Fellow of the American Academy of Microbiology and has received several awards for his outstanding teaching skills, including the 1997 Carski Foundation Distinguished Teacher Award. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.
Solving Identity Crisis in Animal Cells; Best Practices with DNA Barcodes and STR Analysis
Speakers: Yvonne Reid, Ph.D., Manager, Scientist, ATCC
Jason Cooper, M.S., Adjunct Professor, Community College of Allegheny County
Recorded March 31, 2016
Abstract: Animal cell lines are important in vitro systems and tools for scientists in diverse disciplines such as basic cell biology, genetic mapping, gene expression, and gene therapy. Animal cell line identity, which is a crucial first step in cell line authentication, is frequently underappreciated and ignored by most research scientists. Over the years numerous cell lines have been shown to be misidentified due, in part, to poor techniques and inadequate authentication protocols. It is estimated that the financial loss incurred by misidentified cell lines is in the millions of dollars. Animal cell line identity now requires a comprehensive strategy that employs several complementary technologies. Technological advances have given rise to improved capabilities. An overview of these technologies to include STR analysis for human cell line identity and CO1 for animal cell line identity will be presented.
- Cell line authentication is critical to meet funding, quality control, and publication requirements
- Short tandem repeat (STR) profiling is an accepted form of authentication that differentiates human cell lines
- DNA barcoding complements STR profiling by providing species-specific identification
Biographies: Yvonne A. Reid, Ph.D., is a Manager, Scientist at ATCC. Dr. Reid has been at the forefront of developing and standardizing methods to characterize cell lines by enzymology, karyotyping, and more recently DNA profiling, and to propagate, cryopreserve, and track validated batches of thousands of animal cell lines and strains. These validation protocols have been adopted by cell banks around the world. In addition, Dr. Reid has been heavily involved in developing guidelines for authenticating cell lines and cell types.
Jason Cooper, M.S., is a Biology Professor (Adjunct) at the Community College of Allegheny County in Pittsburgh, PA and a Science Writer at Thermo Fisher Scientific. He has worked as a scientist, researcher, and supervisor at Roche Molecular Systems, Aptagen, Inc., as well as ATCC. Most recently, Mr. Cooper has been pivotal in the development and adoption of the consensus standard ASN-0003 for interspecies cell line identity, based on DNA barcoding. He is currently working on a new standard for species level identification and cross-contamination screening in animal cells by multiplex PCR. Download Presentation (.pdf) View Recording.
2015 Excellence in Research Series
Neural Progenitor Cells – Potent Models of Normal and Disease Neurobiology
Brian A. Shapiro, Ph.D., Technical Writer, ATCC
Recorded November 19, 2015
In vitro neurological research presents many challenges due to the difficulty in establishing high-yield neuronal cultures as well as batch-to-batch consistency. Human induced pluripotent stem cells (iPSCs) have a high expansion capacity and can differentiate into neurological cells types; thus, these cells hold great promise for both regenerative medicine and drug discovery. This webinar will discuss neural differentiation and touch on its roles in investigating neurodegenerative diseases and screening therapeutics. Methods for generating large quantities of neural progenitor cells (NPCs) from human fibroblast-, CD34+-, and Parkinson’s disease-derived iPSCs will then be highlighted. Finally, the generation and function of NPC reporter cell lines, created using zinc finger nuclease gene editing technology, will be examined. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.
How Does ISO 17025 Accreditation Build International Confidence?
Bill Hirt, Ph.D., Director of Accreditation, ANAB
Recorded November 12, 2015
We often speak of the global technical community in the abstract, but accreditation to ISO 17025 makes each accredited laboratory feel part of the practical everyday operation of that community. With our ever-growing science and technologies, manufactured and agricultural materials, telecommunications, internet, cyber-security, weapons, explosives, drugs, medicines, and our homeland security operations and interdiction testing, we need to have confidence that materials are sampled well, tested appropriately, and that their measurement tools are well calibrated. The oversight and coordination of all calibration and testing is standardized and managed as best as possible through ISO, through ILAC, and in the everyday, through labs accredited to ISO 17025. Download Presentation (.pdf) View Recording.
Phenotypic Characterization and Quality Control of Cells from Bacteria to Human Cells
Barry R. Bochner, Ph.D., CEO & CSO, Biolog, Inc.
Recorded October 29, 2015
Phenotype MicroArray (PM) technology allows a biologist to test thousands of phenotypes of a cell line in a single experiment to gain a comprehensive overview of the metabolism, physiology, and pathway fluxes. It provides phenomic and metabolomic information that is complementary to genomic or proteomic analysis and often more easy to interpret and more useful. The PM technology platform is applicable to a wide range of cells including bacterial, fungal, or animal and enables metabolic analysis in the context of genotype-phenotype studies. For example, it can be used for (1) analyzing cells with mutations to determine the metabolic and physiologic effects of genetic differences, (2) studying and defining cell metabolism and metabolic regulation, (3) understanding the interplay of environment and hormonal signals on cell metabolism and physiology, (4) optimizing cell culture conditions in bioprocess development and optimization, and (5) examining and maintaining the stability of cell lines. Specific examples and discoveries will be presented to illustrate the many uses of this cell phenotyping technology. Download Presentation (.pdf) View Recording.
Implementation of the VITEK®-MS and its Use in Microbial Identification
Katalin Kiss, Ph.D., Manager, Scientist, Laboratory Testing Services, ATCC
Recorded October 1, 2015
The rapid identification of microorganisms is a great advantage for industrial and clinical laboratories. In the 150 year history of microbial identification, many techniques have been used to authenticate microorganisms, including biochemical analyses, sequencing, and functional testing. The techniques vary in turnaround time, complexity, accuracy, and specificity. The VITEK®-MS is a recent addition to the arsenal of identification technologies. It brings to the field a very simple user interface, rapid turnaround times, and flexibility in expanding a database to suit the user’s needs. In this webinar, we will discuss current methods of microbial identification and how VITEK®-MS compares to these methods with regard to complexity, turnaround time, sample preparation, and ease of interpretation. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.
STR Profiling for Human Cell Line Authentication
Yvonne A. Reid, Ph.D., Manager/Scientist, Cell Biology
Recorded September 10, 2015
Over the past few decades, much has been written about the misidentification of cell lines, primarily human cell lines. It is not unusual that studies of the ‘same’ cell lines performed by different laboratories often show different results using the same methodology. Thus far, numerous cell lines are known to be misidentified due, in part, to a lack of adequate testing. The financial loss incurred by using misidentified cell lines is estimated in the millions of dollars. Cell line authentication is crucial; however, the scientific community seems indifferent about the consequences or wanting to take steps in preventing it. The validity of scientific data demands that consistent and unequivocal verification of cell line identity is precise. To alleviate these concerns, the implementation of best tissue culture practices to include routine authentication of human cell lines by STR profiling is highly recommended by funding agencies, journal editors, and scientific societies. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.
Seeing is Believing – Reporter-Labeled Microbial Control Strains
Jodie Lee, M.S., Lead Biologist, ATCC
Recorded May 21, 2015
Experimental controls are essential for establishing that protocols, methods, and instruments are functioning correctly and efficiently. ATCC, a world leader in high-quality biological controls, now offers reporter-labeled microbial control strains, including Escherichia coli, Shiga toxin-producing E. coli (STEC), Pseudomonas aeruginosa, Salmonella enterica, and Shigella flexneri. These labels allow for easy, visual discrimination between control strains and sample strains to minimize errors caused by cross-contamination in the laboratory. In this webinar, we will discuss the development and evaluation of reporter-labeled controls, and will examine their applications in microbial detection and quantification, microscopy, host-pathogen interaction studies, and food testing. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.
ATCC Transfection Reagents – Powerful Tools to Enable Genetic Manipulation
James Clinton, Ph.D., Scientist, ATCC
Recorded April 2, 2015
Transfection technology allows for transient genetic manipulation in cell cultures that is quick, reliable, and minimally toxic. Lipid-based transfection reagents are powerful tools to investigate the role of various genes in cellular physiology. Gene expression may be enhanced by delivering DNA or mRNA for specific genes or gene products into cells. In addition, gene knockdown may be achieved through transfection of small RNA constructs, which utilize the RNAi pathway to inhibit gene expression. This webinar will provide an introduction to transfection, an overview of current transfection approaches, and a brief guide to best practices in the design and optimization of transfection experiments. Critical aspects include the cell type, culture conditions, and design of nucleic constructs. We will then focus on ATCC’s highly efficient transfection reagents, which have been optimized in a broad spectrum of cell types for a range of applications, including gene expression and siRNA-mediated knockdown. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.
Microbiology Quality Control as Described in the Compendia
Scott Sutton, Ph.D., Principal, Microbiology Network, Inc.
Liz Kerrigan, Director, Product Development, Sales and Marketing, ATCC
Recorded March 19, 2015
The quality control microbiology lab serves a valuable function in the pharmaceutical manufacturing environment in terms of providing information on the biological quality of the products being produced. The major tests are familiar to us all – Microbial Limits, Sterility Tests, and Antimicrobial Effectiveness Tests. All of these tests require strict attention to detail for reproducible results, and the compendium also provides guidance on the quality control aspects of laboratory operations. In the first half of this presentation, Dr. Sutton will provide a brief overview of both of these aspects of microbiological quality control with guidance for the listener on how to further research best practice as described in the compendium. In the second half, Mrs. Kerrigan will describe how ATCC is striving to meet the current needs of the quality control microbiology lab. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.
Enhancing Vector-borne Research with Biological and Molecular Standards
David Clawson, M.S., Lead Biologist, ATCC
Recorded March 5, 2015
Vector-borne diseases are a major public health concern, affecting billions of people worldwide. Due to the complexity of vector-borne pathogen transmission, these illnesses are among the most difficult infectious diseases to predict, prevent, and control. Moreover, many vector-borne pathogens can be challenging to culture, require high-containment facilities, or are on the commerce control list, making them difficult to study. To support the development of rapid diagnostic tools and innovative therapeutics, ATCC has synthetically derived nucleic acids that represent key target regions from a number of infectious microorganisms, including dengue virus, West Nile virus, and Eastern equine encephalitis virus, among others. These standards are quantitated, stable, can be handled in BSL-1 conditions, and don’t require permits for international shipping. In this webinar, we will discuss emerging vector-borne microbial pathogens, molecular and biological products from ATCC that support vector-borne research, and will demonstrate the use of ATCC synthetic molecular standards microbial detection and quantification. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.
Discovering ATCC Hematopoietic Progenitor Cells – Model Systems to Study the Immune and Cardiovascular Systems
James Clinton, Ph.D., Scientist, ATCC
Recorded February 19, 2015
The cellular components of blood originate in bone medullary cavities. In the process of becoming fully functional, hematopoietic cells undergo a program of differentiation which begins in the marrow and may be completed in the peripheral tissues and organs such as blood, lymph, thymus, and spleen. The result is a diversity of cell types, each of which displays specific transport, hemostatic, and immune functions. Hematopoietic research tools have high value for investigating the pathogenesis of anemia and autoimmune diseases, and are useful controls in liquid tumor studies. In this webinar, ATCC scientists will discuss recent developments in developing models of hematopoiesis using immunological cells such as CD34+ bone marrow cells, CD14+ peripheral blood monocytes, and primary peripheral blood mononuclear cells. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.
Accuracy Matters – Proficiency Testing Programs Offer Dependable Materials for Assessments with Trackable Online Results
Tracey Noblett, M.B.A., Head of Microbiology, Proficiency Testing, LGC and
Matthew Whetton, Ph.D., Head of Chemistry, Proficiency Testing, LGC
Recorded February 5, 2015
LGC is a leading life sciences measurement and testing business that offers a comprehensive range of products and services, including proficiency testing programs from LGC’s custom-built facility in the north of England. This presentation will discuss the general requirements and purpose of proficiency testing with reference to ISO/IEC 17043:2010. There will also be a brief overview of the different programs and samples offered by LGC, and available from ATCC in the U.S., including program logistics, sample preparation and quality control, and the statistical assessment and reporting of results. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.
2014 Excellence in Research Series
3D Tissue Modeling
John Pulliam, Ph.D., Field Application Scientist, ATCC
Recorded November 13, 2014
The emergence of 3D tissue modeling raises new possibilities for the study of complex physiological processes in vitro. Advances in cell isolation, media development, substrates, and growth surfaces are leading to protocols that provide more functionality than traditional 2D cell culture. These models may provide a more predictive analysis and result in a more streamlined process of drug discovery and development. In this webinar, we will discuss recent developments in 3D modeling using ATCC primary and hTERT immortalized cells in areas such as angiogenesis, wound healing, and respiratory studies. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording
Precise Counting of Targeted Nucleic Acids has Never Been Easier
Francisco Bizouarn, Global Digital Applications Specialist, Digital Biology Center, Bio-Rad Laboratories
Recorded October 30, 2014
Droplet Digital PCR (ddPCR) is an elegant technology that permits accurate and absolute quantification of target nucleic acid molecules. By subpartitioning a PCR reaction into thousands of nanoliter sized droplets, amplifying, and subsequently individually interrogating them, ddPCR allows for increased quantitative resolution, enhanced detection of difficult to amplify targets, better reproducibility, and simplified data analysis. This webinar will provide a technical overview of ddPCR from technical and workflow related perspectives as well as review key applications such as copy number variation (CNV) analysis, rare mutation detection (RMD) analysis, and standard free (stand-alone) absolute quantification of nucleic acids. Download Presentation (.pdf) View Recording
Using LUHMES Cells as a Model System to Study Dopaminergic Neuron Cell Biology
Tigwa H. Davis, Ph.D., Senior Scientist, Neurobiology, ATCC
Recorded October 16, 2014
Dopaminergic neurons play significant roles in motor, reward, and motivational behavior related circuits throughout the brain. To date, there are few continuous in vitro models available to laboratories in research, industry, and academia for studies related to basic dopaminergic cell biology or high throughput screening. Here, we propose the use of a human model system, LUHMES cells, to study dopaminergic neuron cell biology. During this webinar, we will highlight some of the advantages of using LUHMES cells, as well as examples of how they have been used in drug screening and to study the molecular mechanisms related to Parkinson’s Disease. Download Presentation (.pdf) View Recording
ATCC® Genetic Alteration Cell Panels: Effective Tools for High Throughput Screening Using Corning® Epic® Technology
Fang Tian, Ph.D., Lead Scientist, ATCC
David H. Randle, Ph.D., Manager, Applications Development, Corning Life Sciences
Recorded September 18, 2014
Extensive genomic™ sequencing efforts in recent years have provided detailed profiles of the somatic gene mutations that occur in a wide range of human cancers. In order to facilitate basic and translational cancer research, ATCC has designed and validated a number of Genetic Alteration Cell Panels targeting the key molecular pathways identified in these studies. To demonstrate suitability of the panels for high throughput screening, the EGFR panel was selected for evaluation using Corning Epic Technology, a label-free platform that uses optical biosensors for high sensitivity biochemical and cell-based assays. In this webinar, we will discuss how the combination of Epic Technology and the EGFR Genetic Alteration Panel offers convenient tools to screen for ligands or biologics that directly target or affect EGFR receptor biology. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording
ATCC® Influenza Research Materials
Shamaila Ashraf, M.Sc, M.Phil, Ph.D., Senior Scientist, ATCC
Recorded September 11, 2014
Influenza remains one of the most significant infectious diseases worldwide, causing acute respiratory tract illnesses and accounting for 25% of infections that exacerbate chronic lung infections. To date, several epidemics and four major influenza pandemics have been recorded. Influenza viruses have caused an estimated 3 million cases of serious illness and around 500,000 deaths annually worldwide. Influenza infections are primarily and effectively controlled by vaccines that elicit protective immunity. Influenza viruses undergo rapid antigenic shift and drift that results in the emergence of new strains each year. Therefore, influenza vaccines need to be reformulated every year to match the circulating strains. In this webinar, we will provide an overview of the influenza virus and will explore the current treatment strategies for influenza infections. We will also highlight viral strains and associated materials offered by ATCC that can be used in influenza research or in the development and validation of novel preventative and therapeutic techniques. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording
ATCC® Human Pluripotent Stem Cells – Enabling Research through Standards
Yukari Tokuyama, Ph.D., Field Application Scientist, ATCC
Recorded August 21, 2014
Induced pluripotent stem cells (iPSCs) provide a powerful tool to model human disease in relevant cell types. iPSCs may be generated from patients of any genetic background, and possess the capacity to differentiate into almost any desired terminal cell type. In this webinar, we will focus on ATCC's approach in generating and providing standardized, quality controlled, and highly characterized human iPSCs lines with its complete culture systems. We will also highlight the characterization of LRRK2 Parkinson’s patient-derived iPSC lines by whole-exome sequencing. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording
STR DNA Profiling – The Standard for Cell Line Authentication
Douglas R. Storts, Ph.D., Head of Research - Nucleic Acid Technologies, Promega Corporation and
Yvonne A. Reid, Ph.D., Manager/Scientist, Cell Biology Program, ATCC
Recorded June 5, 2014
Misidentification of human cell lines is a common problem that leads to irreproducible research in the Life Sciences. Over the years, use of contaminated cell lines has increased due to poor techniques, inadequate authentication protocols, and sharing of false cell lines among researchers. These concerns can be readily resolved by the implementation of the Standard STR protocol –authentication of human cell lines by STR profiling. In this webinar, we will discuss the recent advances in STR profiling technologies and will delve into further detail on how the Standard STR protocol is transforming scientific practices. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording
The Importance of Standards in Molecular-Based Assays
Liz Kerrigan, Director, ATCC Standards
Recorded May 8, 2014
Stimulated by the presence of emerging and re-emerging microbial threats, the need for molecular-based tests has significantly increased in the last few years. In turn, this has necessitated the use of standardized nucleic acids in clinical laboratories. In this webinar, we will discuss the importance of molecular standards in the establishment of sensitivity, linearity, and specificity during assay validation or implementation, quality assurance, quality control, and proficiency testing, and how the use of standards can contribute to improvements in assay reproducibility and reliability. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording
ATCC Breast Cancer Research Resources
Fang Tian, Ph.D., Lead Scientist, Cell Biology Group Leader, ATCC
Recorded April 24, 2014
Breast cancer is the leading cause of cancer-related mortality in women. New disease classifications, relevant signaling pathways, and genetic regulators of breast cancer have been identified over the past decade. To continue facilitating progress in basic research and drug discovery, ATCC provides breast cancer research resources. In this webinar, we will discuss triple negative breast cancer cell lines, breast cancer mouse models, and commonly used breast cancer in vitro models with in-depth genetic alteration and molecular profiles. We will also highlight cell lines that can be used to address recently identified genomic and clinical features of breast cancer subtypes. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording
hTERT Immortalized Cell Lines – Unique Tools for Physiologically Relevant Research
Chengkang Zhang, Ph.D.
Recorded March 27, 2014
Human telomerase (hTERT) immortalized cell lines combine the properties of primary cells and the long culture life of continuous cell lines. In this webinar, we will provide an overview of the ATCC hTERT Immortalized cell line collection, and will examine the use of immortalized renal epithelial cells, keratinocytes, and microvascular endothelial cell lines to demonstrate how hTERT immortalized cell lines can help investigators reach their goals. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording
Drug-Resistant Acinetobacter baumannii – A Growing Superbug Population
Cara Wilder, Ph.D., Technical Writer, ATCC
Recorded March 13, 2014
In recent years, natural and societal selective pressures have led to the emergence of numerous antibiotic-resistant microbial strains, including multidrug- and pandrug-resistant strains of Acinetobacter baumannii. These strains have become a significant cause of nosocomial infection among immunologically compromised individuals, resulting in increased morbidity and mortality. This presentation will provide an overview of this escalating problem and will explore the current techniques used to identify drug-resistant A. baumannii strains, available therapies, and remaining concerns. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording
Authentication and Characterization of Animal Cell lines: Towards Best Practices in Cell Culture
Yvonne A. Reid, Ph.D., Manager/Scientist
Cell Biology Program, ATCC
Recorded February 27, 2014
Over the years, numerous cell lines have been shown to be misidentified due in part to poor techniques, inadequate authentication protocols, and sharing of unauthenticated cell lines amongst researchers. Technological advances have given rise to improved capabilities. Cell line authentication and characterization now requires a comprehensive strategy that employs several complementary technologies for systematic testing for morphology, microbial contaminations, cellular cross-contamination, as well as functionality. An overview of the current technologies used to authenticate and characterize animal cell lines will be presented. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording
ATCC – Sophisticated Approaches to In Vitro Research
John Pulliam, Ph.D., Field Application Scientist, ATCC
Recorded February 6, 2014
This webinar will provide an overview of the services that ATCC offers to the scientific community. We will also discuss the sophisticated approaches of utilizing Genetic Alteration Panels; cell lines which are grouped by tissue type or pathway mutation, hTERT immortalized cells, and the HEKPlus system for protein production. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording
2013 Excellence in Research series
ATCC Molecular Signature Panels – Powerful tools for the genomics age
Fang Tian, Ph.D., Lead Scientist, Cell Biology Group, ATCC
Recorded December 5, 2013
Want to bring your research into the genomics age? In this webinar, we will describe the ATCC molecular signature panels, which were generated by combining together authenticated cell lines that contain critical gene copy number changes and site mutations identified by next-generation sequencing. These panels focus on key components of cell signaling pathways. The gene expression, protein expression and cellular localization of EGFR, AKT, PI3K, PTEN, p53, RAS, RAF, ERK, MYC and MET were studied and compared within a large number of cell lines with various genetic backgrounds. View Recording
Breathing rocks for fun and profit
Jeffrey A. Gralnick, Ph.D., Associate Professor of Microbiology, University of Minnesota
Recorded November 14, 2013
Some bacteria have the ability to live by breathing certain oxide minerals. I will talk about one of the best understood model organisms for this process, Shewanella oneidensis, and how a detailed understanding of this microbe could lead to breakthroughs in the areas of energy and bioremediation. View Recording
ATCC Transfection Reagents - A better style of protein expression!
Recorded October 24, 2013
Transfections got you down? It’s true - some cells are harder to transfect than others, but ATCC is here to help. This webinar will describe how transfection reagents and culturing practices can affect the success of your experiments. In addition, we will demonstrate how our new transfection reagent, TransfeX, can help you transfect even the most notoriously difficult cells (i.e. stem cells, primary cells) and help you get your experiments going. View Recording
Mycoplasma Detection – Protect your continuous cell cultures
Cara Wilder, Ph.D., Technical Writer, ATCC
Recorded October 10, 2013
Are you sure your continuous cell cultures and media are free from contamination? Mycoplasma contamination affects roughly 15-35% of continuous cell cultures, resulting in deleterious effects including the induction of chromosomal abnormalities, the disruption of DNA and RNA synthesis, and the inhibition of both cell metabolism and growth rate. In this presentation, learn how to protect your cultures using the ATCC® Mycoplasma Detection Kit. Download Q&A Session (.pdf) View Recording
Primary Cell Solutions - Biologically relevant in vitro models
Recorded September 26, 2013
Did you know, ATCC, your trusted source for tumor cell lines, also offers primary cells? In this webinar, we will describe how our broad offering of primary cells, optimized media systems and transfection reagents, can help you get your primary cell cultures up and running, so you can get your experiments going, and your research moving forward. View Recording
ATCC Toolbox—Kick-start your semester!
Cara Wilder, Ph.D.,Technical Writer, ATCC
Recorded September 12, 2013
The academic year has begun, but are you ready for it? Let ATCC help you set up your lab! In this presentation, we will discuss some of our products designed with teaching laboratories in mind. We will also provide helpful information and key features about our Culture Guides and the new Introduction to Microbiology Guide. Get your students on the fast track to success! Download Q&A Session (.pdf) View Recording
Fungal disease, a 21st century challenge to bat conservation
David Blehert, Ph.D., Microbiologist, U.S. Geological Survey,
National Wildlife Health Center
Recorded June 13, 2013
Since first discovered in 2007 near Albany, New York, bat white-nose syndrome has spread to 20 US states and five Canadian provinces, and the disease is estimated to have killed over five million insectivorous hibernating bats. An outbreak of infectious disease among bats on the order of white-nose syndrome is without precedent. This presentation will provide an overview of this novel emergent wildlife disease and explore the profound impacts it may have on bat conservation in the 21st century. View Recording
Stem Cell Solutions
John Pulliam, Ph.D., Marketing Application Scientist, ATCC
Recorded June 6, 2013
ATCC is your trusted source for induced pluripotent stem cell (iPSC) technology! This webinar will provide background on human iPSC and ATCC products and introduce the advantages of our new human iPSC products. Finally, we will demonstrate helpful tips and solutions for using the ATCC feeder-free human iPSC culture system for the thawing, passaging and cryopreservation. View Recording
Introducing your new prokaryotic species
Recorded May 16, 2013
For over 85 years, ATCC has been helping scientists store and distribute their prokaryotic strains. In that time we have developed considerable expertise in maintaining the strains deposited with us, but it is up to the depositor to name their species and ensure that the name is recognized by the research community. In this talk, we will go over the deposit and name validation process, providing tips and pointing out potential bumps in the road. You’ll also get a list of essential publications and useful web sites to help you make sure your new species is successfully introduced to the world. Resources for Nomenclature and Taxonomy (.pdf) Download Q&A session (.pdf) View Recording
Carbapenem-Resistant Enterobacteriaceae (CRE) – A Growing Superbug Population
Recorded April 18, 2013
In recent years, natural and societal selective pressures have led to the emergence of numerous antibiotic-resistant microbial strains, including carbapenem-resistant Enterobacteriaceae (CRE) expressing Klebsiella pneumoniae carbapenemase (KPC) or New Delhi metallo-β-lactamase (NDM-1). Infection with these strains has become a leading cause of nosocomial infections among immune-compromised individuals, resulting in increased morbidity and mortality. This presentation will provide an overview of this escalating problem and will explore the current techniques used to identify CRE strains, available therapies, and remaining concerns. View Recording
2012 Excellence in Research series
Non-O157 Shiga Toxin-Producing Escherichia coli
Recorded November 15, 2012
The global community has been experiencing food-associated outbreaks of non-O157 Shiga toxin-producing Escherichia coli (STEC) for nearly two decades. As a result, six non-O157 STEC strains were classified as adulterants by the Food Safety and Inspection Service branch of the U.S. Department of Agriculture in June 2012, requiring routine verification testing of raw beef manufacturing trimmings for serogroups O26, O45, O103, O111, O121, and O145. ATCC has recently accessioned representative strains (both toxigenic and non-toxigenic) for each serogroup from the CDC and now has them available for use as controls for diagnostics and process validation. This webinar will discuss the genetic and phenotypic methods used to characterize these strains. Download Q&A session (.pdf) View Recording
Updates to the ATCC Material Transfer and Deposit Agreement: How ATCC Helps You in Research
Recorded October 18, 2012
Earlier this year, ATCC revised its Material Transfer Agreement, to enable researchers and external research sites to transfer key biological reagents derived from ATCC material, such as transfected cell lines and genomic DNA, to collaborators without seeking permission from ATCC or paying administrative transfer fees. Additionally, the ATCC Deposit Agreement has been updated to allow those depositing with ATCC to share in the financial success of a deposit by receiving a royalty stream, once a certain sales threshold has been reached. In this webinar, we will provide more details about these updated agreements, and address how these changes affect the research community. Download Q&A session (.pdf) View Recording
Assessing Novel Storage Techniques and Their Ability to Stabilize Nucleic Acids
Kurt Langenbach, PhD, Manager, Research Scientist, Cores, ATCC and
Maria Mayda, Scientist, Assay Development, ATCC
Recorded October 4, 2012
Novel ambient temperature stable storage approaches offer the potential advantage that various materials, such as nucleic acids, could be subjected to a broad range of hostile temperatures without diminishing and perhaps even improving their performance in downstream applications. In this webinar, we will discuss some of the results generated through our technology assessment program that have focused on evaluating innovative methods for the collection, processing, preservation, and characterization of bio-specimens, including nucleic acids, at ambient temperature. Insights gathered from these studies will allow us to move beyond traditional biological sample storage concepts, enhance the quality and reproducibility of results, improve sample handling, and minimize the impact of deleterious events in cold chain management. Download Q&A session (.pdf) View Recording
Biological Resources of the ATCC Protistology Collection
Robert Molestina, PhD, Senior Scientist, ATCC
Recorded September 6, 2012
Protists are eukaryotic microorganisms commonly studied due to their ecological relevance in aquatic food chains and their impact on human health. Protistology research and comparative studies are dependent on the accessibility of authenticated biological standards. To meet this need, the Protistology Collection at the ATCC houses the largest and most diverse repository of protist cultures in the world. The primary role of the collection has been the acquisition, authentication, preservation, and distribution of reference strains. Characterization of deposited cultures includes a variety of tests such as viability, purity, phenotypic properties, and genotypic analysis. Additionally, over the last decade, resources within the collection have expanded to include genomic DNA and custom services. This presentation will provide an overview of biological resources available in the ATCC Protistology Collection, a discussion on the methods of characterization, benefits of depositing, current research projects, and future perspectives. View Recording
Tumor Cell Panels in Cancer Research and Drug Discovery
Fang Tian, Senior Scientist, Cell Biology, ATCC
Recorded August 23, 2012
Human cancer cell lines have been used as essential tools in both high-throughput drug screening and detailed molecular mechanism studies. This Webinar will focus on genetic characterization of human cancer cell lines currently used in laboratories. Additionally, it will introduce how to choose the best cell lines and cell line panels for biological understanding of top genetic alterations across tumor types, and for testing small molecules or biologics for cancer drug development. View Recording
Good Cell Culture Practices
Misidentification/cross-contamination, long-term subculturing and passage number, poor culture conditions and microbial contamination (mycoplasma). View Recording
- Free online cell culture and assay training webinars.
You are invited to a series of free web-based technical seminars on cell culture. Co-sponsored by ATCC and The Society for In Vitro Biology (SIVB), the webinars are designed to provide novel tips, best practices and proven techniques to help with cell culture research needs.