Introduction to ATCC® Microbiome Standards

An End-To-End Solution for the Standardization of Microbiome Research

9/7/2017


Abstract:

The complexities involved in 16S rRNA and shotgun metagenomic analysis methods pose significant challenges for microbiome research. Significant biases can be introduced during PCR amplification, library preparation, sequencing, and analysis. One of the primary obstacles in assay standardization is the limited availability of reference materials. To support this need, ATCC has developed microbiome reference standards from fully sequenced and characterized ATCC strains. To further enhance the use of these standards, ATCC has collaborated with One Codex to develop an easy-to-use data analysis module that enables researchers to analyze both shotgun and 16S rRNA data quickly and accurately while eliminating the biases associated with data analysis. In this webinar, we will discuss the need for standards in microbiome research, the development and use of the ATCC® Microbiome Standards, and how to analyze these standards using the One Codex data analysis platform.

Key Points:

  • One of the primary obstacles in the standardization of microbiome assays is the limited availability of reference materials
  • ATCC has developed microbiome reference standards from fully sequenced and characterized strains for use as daily run controls and in assay development
  • To eliminate bias associated with data analysis, ATCC has collaborated with One Codex to combine the power of physical laboratory standards with the leading bioinformatics platform for microbial genomics

Presenters

Dev Mittar

Dev Mittar, Ph.D.,
Lead Scientist, ATCC

Dev Mittar, Ph.D., is a Lead Scientist and the head of the microbiology R&D group at ATCC. At ATCC, He has been instrumental in the development of controls and standards for various infectious microorganisms with applications in the area of molecular diagnostics and clinical, food, and pharmaceutical microbiology. He led the development of ATCC Microbiome standards with a goal to help microbiome researchers standardize their methods. Dr. Mittar received post-doctoral training at Weill Medical College of Cornell University and worked at BD Biosciences before joining ATCC.

Nick Greenfield

Nick Greenfield, M.A.,
Founder & CEO, One Codex

Nick Greenfield, M.A., is the founder and CEO of One Codex, a microbial genomics software company based in San Francisco, California. One Codex is the leading microbial genomics and metagenomics platform, and has received awards from the Centers for Disease Control (CDC) and Department of Defense (DoD) as well as been recognized by the White House National Microbiome Initiative. At One Codex, Nick leads the development of the company's bioinformatics and software technology, and oversees the company's efforts in application areas including infectious disease diagnostics, microbiome R&D, and public health. Prior to One Codex, Nick worked as a software engineer and data scientist on a variety of machine learning and scientific computing problems. Nick holds a B.A. in International Relations and a M.A. in Environmental Studies from Brown University.