HTB-132 cells can be slow to attach and may produce large amounts of floating cells and debris. It is normal to see many floating cells during the first few days after initiation. It is important not to discard these cells during media changes because most cells are viable and will eventually attach to the flask and grow. The floating cells should be retained by gentle centrifugation and added back to the adherent population.
Adherent MDA-MB-468 cells are small and may be multinucleated. Debris tends to become heavier as the monolayer grows to confluence. It is important to be aware that the ATCC’s Leibovitz's L-15 Medium, ATCC® 30-2008 ™ is devised for use in free gas exchange with atmospheric air setup. A CO2 and air mixture is detrimental to cells when using this medium for cultivation
|Date Created||02/21/2014 11:29 AM
|Date Updated||02/21/2014 11:30 AM