5-bromo-2’-deoxyuridine (variously abbreviated as BrdU, BUdR, BrdUrd) is used in the medium for ATCC® CRL-8303 ™ to maintain selection pressure on the culture.
ATCC® CRL-8303 ™, 143B, is a Thymidine Kinase negative [TK(-)] cell line. Cells lacking TK can be selected by resistance to bromodeoxyuridine. Cells that are TK(+) are not resistant bromodeoxyuridine and will be killed in the presence of the BrdU.
|Date Created||08/19/2013 09:43 AM
|Date Updated||08/19/2013 10:19 AM