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Adding 5-bromo-2’-deoxyuridine to CRL-8303 medium

Why is it necessary to add 5-bromo-2’-deoxyuridine to the complete growth media for ATCC® CRL-8303 ™?

5-bromo-2’-deoxyuridine (variously abbreviated as BrdU, BUdR, BrdUrd) is used in the medium for ATCC® CRL-8303 ™ to maintain selection pressure on the culture.

ATCC® CRL-8303 ™, 143B, is a Thymidine Kinase negative [TK(-)] cell line.  Cells lacking TK can be selected by resistance to bromodeoxyuridine.  Cells that are TK(+) are not resistant bromodeoxyuridine and will be killed in the presence of the BrdU.

 

Date Created08/19/2013 09:43 AM
Date Updated08/19/2013 10:19 AM

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