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Karyotype of ATCC® HTB-55™

My lab uses Calu-3, an adenocarcinoma cell line derived from a pleural effusion, that we obtained from ATCC® HTB-55 ™. The cytogenetics data online suggests that normal chromosome 17 is "absent." However, we find the gene encoding NHERF-1, which is situated on chromosome 17, to be strongly expressed in this line. Is the karyotype provided by ATCC for HTB-55 incorrect?

When a karyotype for an ATCC cell line reports "normal" chromosomes are "absent," it usually means that abberrant ones are present. Karyotyping performed for HTB-55 shows this line is highly rearranged. It is very possible, if not likely, that parts of chromosome 17 can be found translocated throughout the genome, which would explain your positive results for NHERF-1 found on chromosome 17. In fact, if the gene was located on a part of the chromosome that was duplicated as a result of it's translocation or insertion, then it may be over-expressed by the cells. Detection of chromosome 17 in HTB-55 will require a 17 paint probe or spectral karyotype (SKY), not currently performed by ATCC for this line.

Date Created07/17/2012 07:14 PM
Date Updated02/07/2013 03:18 PM

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