TIB-152 should be initiated at a seeding density of 1.0 - 2.0 X 105 viable cells /ml. The culture can be maintained by adding small amounts of fresh medium to the flask every 2 or 3 days, and performing frequent cell counts to ensure that the density does not exceed 3 X 106 viable cells/ml. Cultures should then be transferred to a larger flask, or more flasks, as necessary. It is important to dilute the culture with fresh medium to lower the cell concentration and provide sufficient nutrients for growth. If the medium is simply replaced (by spinning down and re-suspending in the same volume) and the cell density is not decreased, the cells can deplete the medium rapidly and die.
|Date Created||02/21/2014 11:51 AM
|Date Updated||02/21/2014 11:52 AM