FAQ Home

Find Answers

Antibiotics in Ker-CT medium
Should I use antibiotics in the culture medium for the Ker-CT cells (ATCC® CRL-4048 ™)? What are the recommended concentrations?

Antibiotics are not recommended as they can mask contamination.

PLEASE NOTE that the Ker-CT cell line (ATCC® CRL-4048 ™) is immortalized by human telomerase and CDK4, using the retroviral pBABE-puro hTERT and pSRalphaMSU plasmid expressing mouse CDK4, hence the cell line is resistant to both puromycin (350 ng/mL) and G418 (100 µg/mL).

Date Created11/19/2013 11:52 AM
Date Updated03/27/2014 08:41 PM

Most Popular Answers

  1. Huh7 cell line
  2. ATCC HUVEC lines
  3. U-373 MG (ATCC® HTB-17)
  4. Passage number vs. population doubling level (PDL)
  5. Converting TCID[50] to plaque forming units (PFU)