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Antibiotics in Ker-CT medium
Should I use antibiotics in the culture medium for the Ker-CT cells (ATCC® CRL-4048 ™)? What are the recommended concentrations?

Antibiotics are not recommended as they can mask contamination.

PLEASE NOTE that the Ker-CT cell line (ATCC® CRL-4048 ™) is immortalized by human telomerase and CDK4, using the retroviral pBABE-puro hTERT and pSRalphaMSU plasmid expressing mouse CDK4, hence the cell line is resistant to both puromycin (350 ng/mL) and G418 (100 µg/mL).

Date Created11/19/2013 11:52 AM
Date Updated03/27/2014 08:41 PM

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