DNA from Chlamydia trachomatis strain UW-3/Cx (ATCC® VR-885D)

Organism: Chlamydia trachomatis Serovar D  / 

Product Format frozen
Total DNA from infected cells, ≥1 µg/ml in 1X TE buffer
100 µL per vial, shipped frozen
Storage Conditions Store at -70°C or colder
Shipping Information Frozen
Applications
DNA isolated from infected cells is appropriate for PCR and other molecular biology applications.
Sexually Transmitted Disease Research
Comments
Chlamydia DNA, in total DNA isolated from McCoy cells (ATCC CRL-1696) infected with Chlamydia trachomatis strain UW-3/Cx (ATCC VR-885). This product was prepared using methods known to inactivate the infecting agent. DNA isolated from infected cells is appropriate for PCR and other molecular biology applications.
Focus Area
Infectious diseases
Biosafety Level 1
Quality Control Specifications
  1. Store at -20°C or colder. -70°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).
  2. Concentration is verified as ≥1 µg/mL DNA by Picogreen® assay.
  3. Purity is confirmed by OD260/OD280 ratio (≥1.7) of bulk DNA before dilution for product filling.
  4. Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis of 20 µl of product on a 0.8% agarose gel, visualized by Ethidium bromide staining.
  5. Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using Chlamydia-specific primers.
  6. Identity is confirmed by sequencing of approximately 300 bp to 1Kb of the PCR amplicon.