Human Coxsackievirus B3 (ATCC® VR-30)

Classification: Picornaviridae, Enterovirus, Human enterovirus B  /  Product Format: frozen 1 mL per vial

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Classification Picornaviridae, Enterovirus, Human enterovirus B
Deposited As Coxsackievirus B3
Agent Human Coxsackievirus B3
Strain Nancy
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Cryopreserved in 10% FBS.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
LLC-MK2 (ATCC® CCL-7.1™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days
Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor JL Melnick
Source Stool from febrile patient with minor illness, Connecticut, 1949
References

Melnick JL, Ledinko N. Immunological reactions of the Coxsackie viruses. I. The neutralization test; technic and application. J. Exp. Med. 92: 463-482, 1950. PubMed: 14778925

Cross References

Nucleotide (GenBank) : AJ295194 Human coxsackievirus B3 genomic RNA for partial polyprotein gene, isolate VR-30, ATCC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation