Human rhinovirus 14 (ATCC® VR-284)

Classification: Picornaviridae, Rhinovirus, Human rhinovirus B  /  Product Format: frozen 1 mL per vial

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Classification Picornaviridae, Rhinovirus, Human rhinovirus B
Agent Human rhinovirus 14
Strain 1059
Applications
Virucide testing
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-284
Effect on Host
CPE, rounding and sloughing
Recommended Host
H1-HeLa (ATCC® CRL-1958™)
Growth Conditions Temperature: 33°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium.
Incubation: 1-5 days while rocking continuously
Effect on Host
CPE, rounding and sloughing
Name of Depositor RM Conant, VV Hamparian, The Ohio State University
Special Collection ATCC
Source Throat swab from young adult with upper respiratory illness, South Carolina, 1959
References

Johnson KM, Rosen L. Characteristics of five newly recognized Enteroviruses recovered from the human oropharynx. Am. J. Epidemiol. 77(1): 15-25, 1963.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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