Human Coxsackievirus B1 (ATCC® VR-28)

Classification: Picornaviridae, Enterovirus, Human enterovirus B  /  Product Format: frozen 1 mL per vial

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Classification Picornaviridae, Enterovirus, Human enterovirus B
Deposited As Coxsackievirus B1
Agent Human Coxsackievirus B1
Strain Conn-5
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Deposited as Coxsackievirus B1. Recognized as prototype strain in Panel for Picronaviruses, National Institute of Allergy and Infectious Diseases at time of deposit.
Effect on Host
CPE, refractile cell rounding and sloughing
Recommended Host
LLC-MK2 Derivative (ATCC® CCL-7.1™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-3 days in a humidified 5% CO2 atmosphere, until CPE are well advance throughout culture.
Effect on Host
CPE, refractile cell rounding and sloughing
Name of Depositor JL Melnick, Yale University School of Medicine
Source Stool from patient with aseptic meningitis, Connecticut, 1948
References

Melnick J, et al. A virus isolated from patients diagnosed as non-paralytic poliomyelitis and aseptic meningitis. Proc. Soc. Exp. Biol. Med. 71: 344-349, 1949. PubMed: 18136475

Cross References

Nucleotide (GenBank) : AJ295196 Human coxsackievirus B1 genomic RNA for partial polyprotein gene, isolate VR-28, ATCC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation