Human Coxsackievirus B4 (ATCC® VR-184)

Classification: Picornaviridae, Enterovirus, Enterovirus B  /  Product Format: frozen 1.0 mL

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Classification Picornaviridae, Enterovirus, Enterovirus B
Deposited As Coxsackievirus B 4
Agent Human Coxsackievirus B4
Strain J.V.B. (Benschoten)
Biosafety Level 2
Isolation
Stool from 10-year-old child, New York, 1951
Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Images ATCC VR-184 images
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
LLC-MK2 Derivative (ATCC® CCL-7.1™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and sloughing
Name of Depositor H Plager
Source Stool from 10-year-old child, New York, 1951
Year of Origin 1951
References

Dalldorf G. The Coxsackie viruses. Bull NY Acad.. Med 26: 329-335, 1950. PubMed: 15411582

Sickles GM, et al. Isolation and type determination of Coxackie virus, group B, in tissue culture. Proc. Soc. Exp. Biol. Med. 88: 22-24, 1955. PubMed: 14357332

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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