Influenza A virus (H1N1) (ATCC® VR-1737)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen 1 mL per vial

Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H1N1)
Strain A/Virginia/ATCC2/2009
Applications
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1737 images
Comments
Item analyzed using Real-time RT-PCR to determine strain is of pandemic swine Influenza A origin.
Effect on Host
CPE, rounded refractile cells with cell degeneration
Recommended Host
MDCK (ATCC® CCL-34™)
Growth Conditions
Temperature: 35°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-5 days
Effect on Host
CPE, rounded refractile cells with cell degeneration
Name of Depositor ATCC
Special Collection ATCC
Source Nasopharyngeal specimen from a clinical patient, 2009