Influenza B virus (ATCC® VR-1735™)

Classification: Orthomyxoviridae, Influenzavirus B  /  Product Format: frozen 1 mL per vial

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Classification Orthomyxoviridae, Influenzavirus B
Agent Influenza B virus
Strain B/Taiwan/2/62
Applications
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1735 images
Comments
Derived by adaptation from allantoic product ATCC® VR-295™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-295™ and ATCC® VR-1735™ have not been compared for sequence or infectivity in chicken embryo culture and tissue culture.
Effect on Host
CPE, rounding and degeneration
Recommended Host
MDCK (ATCC® CCL-34™)
Growth Conditions
Temperature: 33°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days
Effect on Host
CPE, rounding and degeneration
Name of Depositor RQ Robinson, Centers for Disease Control and Prevention; ATCC®
Special Collection ATCC
Source Patient in Taiwan, 1962
References

Green IJ, et al. The isolation and characterization of a new Influenza Type B virus on Taiwan. Am. J. Hyg. 79: 107-112, 1964. PubMed: 14114349

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Micrograph of Host Effects