LLC-Mk2 derivative cells (ATCC CCL-7.1)
Recommended Host: LLC-Mk2 derivative cells (ATCC® CCL-7.1).
Alternate Hosts: MkK, Mk (testis) and HED cells
Plant cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI of about 0.1. Adsorb 1 hour at 37.0°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.